Overview

  • Product name

  • Description

    Rabbit polyclonal to HSPA6
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide from C-terminus of Human HSPA6, conjugated to KLH

  • Positive control

    • Heat shocked HeLa cells
  • General notes

    Concentration: See product label

Properties

Applications

Our Abpromise guarantee covers the use of ab69408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
IP 1/100.

Target

  • Function

    In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.
  • Sequence similarities

    Belongs to the heat shock protein 70 family.
  • Information by UniProt
  • Database links

  • Alternative names

    • Heat shock 70 kDa protein 6 antibody
    • Heat shock 70 kDa protein B' antibody
    • Heat shock 70 kDa protein B'' antibody
    • heat shock 70kD protein 6 (HSP70B') antibody
    • Heat shock 70kDa protein 6 antibody
    • HSP70B' antibody
    • HSP70B-Prime antibody
    • HSP76_HUMAN antibody
    • HSPA6 antibody
    • OTTHUMP00000032372 antibody
    see all

Images

  • All lanes : Anti-HSPA6 antibody (ab69408) at 1/1000 dilution

    Lane 1 : Cell lysates prepared from untreated human Hela cells
    Lane 2 : Cell lysates prepared from heat shock treated (2hrs) Hela cells

    Predicted band size: 71 kDa

References

This product has been referenced in:

  • Gogliotti RG  et al. Total RNA Sequencing of Rett Syndrome Autopsy Samples Identifies the M4 Muscarinic Receptor as a Novel Therapeutic Target. J Pharmacol Exp Ther 365:291-300 (2018). Read more (PubMed: 29523700) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Ich habe jetzt Nachricht aus Labor erhalten, und mir wurde leider mitgeteilt, dass die Ähnlichkeit der Immunogensequenz von ab69408 und ab69551 im Vergleich mit den anderen HSP Proteinen wieder sehr groß ist (sie beträgt bei zB Hsp70 1A/B, Hsp71 über 90%).

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Answer

Vielen Dank für Ihre Anfrage.


ab125024 Kreuzreagiert mit HSP70 1A/B, aber mit keinem anderen Hsp.

Für ab69408 und ab69551 muss ich leider im Labor nachfragen, um die Sequenz zu erhalten.

Sobald ich eine Antwort erhalten habe und den Sequenzvergleich durchgeführt habe, werde ich mich wieder bei ihnen melden.

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