Anti-HspBP1 antibody (ab3858)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-HspBP1 antibody
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DescriptionRabbit polyclonal to HspBP1
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Host speciesRabbit
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SpecificityThis antibody recognises HspBP1 (39kDa) in Western Blots.
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Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
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Species reactivityReacts with: Human
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Immunogen
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Positive control
- This antibody gave a positive signal in HeLa cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab3858 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. | |
| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionInhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
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Tissue specificityUbiquitous.
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Sequence similaritiesContains 4 ARM repeats.
- Information by UniProt
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Database links
- Entrez Gene: 23640 Human
- Omim: 612939 Human
- SwissProt: Q9NZL4 Human
- Unigene: 53066 Human
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Alternative names
- 1500019G21Rik antibody
- FES1 antibody
- Heat shock protein 70 binding protein antibody
see all
Images
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Western blot - Anti-HspBP1 antibody (ab3858)Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution
All lanes : MCF-7 Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39.3 kDa
Observed band size: 39.3 kDa
Exposure time: 2 minutesWestern blot using ab3858 on 20 ug per lane of MCF-7 Whole Cell Lysate. Secondary: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000). Exposure time: 2 mins.
Lane 1: ab3858 at 1/500.
Lane 2: ab3858 at 1/1000.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HspBP1 antibody (ab3858)Image courtesy of Human Protein AtlasParaffin embedded sections of human kidney tissue were incubated with ab3858 (1/75 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6.
ab3858 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org
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Immunocytochemistry/ Immunofluorescence - Anti-HspBP1 antibody (ab3858)ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.
Datasheets and documents
Protocols
References
ab3858 has not yet been referenced specifically in any publications.
