Anti-HspBP1 antibody (ab3858)

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Immunocytochemistry/ Immunofluorescence - Anti-HspBP1 antibody (ab3858)
  • Western blot - Anti-HspBP1 antibody (ab3858)

Key features and details

  • Rabbit polyclonal to HspBP1
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-HspBP1 antibody

  • Description

    Rabbit polyclonal to HspBP1

  • Host species

    Rabbit

  • Specificity

    This antibody recognises HspBP1 (39kDa) in Western Blots.

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Fusion protein corresponding to Human HspBP1.

  • Positive control

    • This antibody gave a positive signal in HeLa cells.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
Notes
WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).
ICC/IF
Use a concentration of 5 µg/ml.

Target

  • Function

    Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Contains 4 ARM repeats.
  • Information by UniProt

  • Database links

    • Alternative names

      • 1500019G21Rik antibody
      • FES1 antibody
      • Heat shock protein 70 binding protein antibody
      • Heat shock protein 70 interacting protein antibody
      • Heat shock protein binding protein 1 antibody
      • Heat shock protein-binding protein 1 antibody
      • Heat-shock 70-KD protein-binding protein 1 antibody
      • HPBP1_HUMAN antibody
      • Hsp 70 binding protein antibody
      • Hsp 70 interacting protein antibody
      • Hsp70 binding protein 1 antibody
      • Hsp70 binding protein 2 antibody
      • Hsp70 interacting protein 1 antibody
      • Hsp70 interacting protein 2 antibody
      • Hsp70-binding protein 1 antibody
      • Hsp70-binding protein 2 antibody
      • Hsp70-interacting protein 1 antibody
      • Hsp70-interacting protein 2 antibody
      • HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 antibody
      • HSPA-binding protein 1 antibody
      • HSPBP antibody
      • HspBP1 antibody
      • HspBP2 antibody
      • PP1845 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-HspBP1 antibody (ab3858)
      Immunocytochemistry/ Immunofluorescence - Anti-HspBP1 antibody (ab3858)

      ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.

    • Western blot - Anti-HspBP1 antibody (ab3858)
      Western blot - Anti-HspBP1 antibody (ab3858)
      Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
      Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution

      All lanes : MCF-7 Whole Cell Lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 39.3 kDa
      Observed band size: 39.3 kDa


      Exposure time: 2 minutes


      Western blot using ab3858 on 20 ug per lane of MCF-7 Whole Cell Lysate. Secondary: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000). Exposure time: 2 mins.

      Lane 1: ab3858 at 1/500.

      Lane 2: ab3858 at 1/1000.

    References (0)

    Publishing research using ab3858? Please let us know so that we can cite the reference in this datasheet.

    ab3858 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Question

    Does this antibody work in IF?

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    Answer

    This antibody has been tested briefly in Immunofluorescence in U20S cells (human). IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. No staining was observed. However, staining was only attempted once and this is by no means an indication that the antibody doesn't work in IF, it merely did not perform under the conditions above. Please see reviews and the datasheet for details of any further testing.

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