• Product name
    Anti-HspBP1 antibody
  • Description
    Rabbit polyclonal to HspBP1
  • Host species
  • Specificity
    This antibody recognises HspBP1 (39kDa) in Western Blots.
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein of Human HspBP1.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in HeLa cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab3858 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39.3 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.


  • Function
    Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR.
  • Tissue specificity
  • Sequence similarities
    Contains 4 ARM repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1500019G21Rik antibody
    • FES1 antibody
    • Heat shock protein 70 binding protein antibody
    • Heat shock protein 70 interacting protein antibody
    • Heat shock protein binding protein 1 antibody
    • Heat shock protein-binding protein 1 antibody
    • Heat-shock 70-KD protein-binding protein 1 antibody
    • HPBP1_HUMAN antibody
    • Hsp 70 binding protein antibody
    • Hsp 70 interacting protein antibody
    • Hsp70 binding protein 1 antibody
    • Hsp70 binding protein 2 antibody
    • Hsp70 interacting protein 1 antibody
    • Hsp70 interacting protein 2 antibody
    • Hsp70-binding protein 1 antibody
    • Hsp70-binding protein 2 antibody
    • Hsp70-interacting protein 1 antibody
    • Hsp70-interacting protein 2 antibody
    • HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1 antibody
    • HSPA-binding protein 1 antibody
    • HSPBP antibody
    • HspBP1 antibody
    • HspBP2 antibody
    • PP1845 antibody
    see all


  • ICC/IF image of ab3858 stained human HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab3858, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in MCF7 cells.

  • Lane 1 : Anti-HspBP1 antibody (ab3858) at 1/500 dilution
    Lane 2 : Anti-HspBP1 antibody (ab3858) at 1/1000 dilution

    All lanes : MCF-7 Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 39.3 kDa
    Observed band size: 39.3 kDa

    Exposure time: 2 minutes

    Western blot using ab3858 on 20 ug per lane of MCF-7 Whole Cell Lysate. Secondary: Goat anti-rabbit IgG HRP conjugate ab6721 (1/2000). Exposure time: 2 mins.

    Lane 1: ab3858 at 1/500.

    Lane 2: ab3858 at 1/1000.

  • Image courtesy of Human Protein Atlas

    Paraffin embedded sections of human kidney tissue were incubated with ab3858 (1/75 dilution) at room temperature for 30 mins. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab3858 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further images can be found at www.proteinatlas.org


ab3858 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


This antibody has been tested briefly in Immunofluorescence in U20S cells (human). IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. No staining was observed. However, staining was only attempted once and this is by no means an indication that the antibody doesn't work in IF, it merely did not perform under the conditions above. Please see reviews and the datasheet for details of any further testing.

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