Product nameHspBP1 overexpression 293T lysate (whole cell)
ab94320 is a 293T cell transfected lysate in which Human HspBP1 has been transiently over-expressed using a pCMV-HspBP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. Tissue specificity: Ubiquitous. Similarity: Contains 4 ARM repeats.
Our Abpromise guarantee covers the use of ab94320 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94320 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-HspBP1 antibody (ab88826) at 1/500 dilution
Lane 1 :
HspBP1 overexpression 293T lysate (whole cell) (ab94320)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 at 1/2500 dilution
ab94320 has not yet been referenced specifically in any publications.