Anti-HSPC111 antibody (ab241590)

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Overview

  • Product name

    Anti-HSPC111 antibody
    See all HSPC111 primary antibodies

  • Description

    Rabbit polyclonal to HSPC111

  • Host species

    Rabbit

  • Specificity

    Antibody was affinity purified using an epitope specific to HSPC111 immobilized on solid support.

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow

  • Immunogen

    Synthetic peptide within Human HSPC111 aa 75-125. The exact sequence is proprietary.
    Database link: Q9Y3C1

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    Antibody was affinity purified using an epitope specific to HSPC111 immobilized on solid support.

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab241590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 21 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • Western blot - Anti-HSPC111 antibody (ab241590)
    Western blot - Anti-HSPC111 antibody (ab241590)
    All lanes : Anti-HSPC111 antibody (ab241590) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 21 kDa


    Exposure time: 30 seconds
  • Immunoprecipitation - Anti-HSPC111 antibody (ab241590)
    Immunoprecipitation - Anti-HSPC111 antibody (ab241590)

    HSPC111 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab241590 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab241590 at 0.4 µg/mL.

    Lane 1: ab241590 IP in HeLa whole cell lysate.
    Lane 2: Control IgG in HeLa whole cell lysate.

    Detection: Chemiluminescence with an exposure time of 30 seconds.

    Lysates prepared in NETN buffer.

     

References

ab241590 has not yet been referenced specifically in any publications.

Publishing research using ab241590? Please let us know so that we can cite the reference in this datasheet.

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