Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11971] to HSPC111
- Suitable for: Flow Cyt, WB, IP
- Reacts with: Mouse, Human
Product nameAnti-HSPC111 antibody [EPR11971]
See all HSPC111 primary antibodies
DescriptionRabbit monoclonal [EPR11971] to HSPC111
Tested applicationsSuitable for: Flow Cyt, WB, IPmore details
Unsuitable for: ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPC111 aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y3C1
- Fetal liver, HeLa, K562 and Raji lysates. Permeabilized K562 cells. Immunoprecipitation pellet from Human fetal liver tissue lysate.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174267 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/1000 - 1/10000. Predicted molecular weight: 21 kDa.|
|IP||1/10 - 1/100.|
Sequence similaritiesBelongs to the NOP16 family.
Cellular localizationNucleus > nucleolus.
- Information by UniProt
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All lanes : Anti-HSPC111 antibody [EPR11971] (ab174267) at 1/1000 dilution
Lane 1 : Fetal liver lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
Flow cytometric analysis of permeabilized K562 cells using ab174267 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver cells lysate or (Lane 2) 1XPBS (negative control) using ab174267 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab174267 has not yet been referenced specifically in any publications.