Recombinant
RabMAb

Anti-HSPC111 antibody [EPR11971] (ab174267)

Overview

  • Product name
    Anti-HSPC111 antibody [EPR11971]
    See all HSPC111 primary antibodies
  • Description
    Rabbit monoclonal [EPR11971] to HSPC111
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, WB, IPmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HSPC111 aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y3C1

  • Positive control
    • Fetal liver, HeLa, K562 and Raji lysates. Permeabilized K562 cells. Immunoprecipitation pellet from Human fetal liver tissue lysate.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR11971
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Sequence similarities
      Belongs to the NOP16 family.
    • Cellular localization
      Nucleus > nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CGI 117 antibody
      • HBV pre S2 trans regulated protein 3 antibody
      • HBV pre-S2 trans-regulated protein 3 antibody
      • HSPC185 antibody
      • Hypothetical protein HSPC111 antibody
      • NOP16 antibody
      • NOP16 Gene antibody
      • NOP16 nucleolar protein homolog antibody
      • NOP16 nucleolar protein homolog (yeast) antibody
      • NOP16_HUMAN antibody
      • Nucleolar protein 16 antibody
      • Nucleolar protein 16 homolog antibody
      • Nucleolar protein 16 homolog (yeast) antibody
      • OTTHUMP00000223274 antibody
      • OTTHUMP00000223277 antibody
      see all

    Images

    • All lanes : Anti-HSPC111 antibody [EPR11971] (ab174267) at 1/1000 dilution

      Lane 1 : Fetal liver lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 21 kDa

    • Flow cytometric analysis of permeabilized K562 cells using ab174267 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

    • Western blot analysis on immunoprecipitation pellet from (Lane 1) Human fetal liver cells lysate or (Lane 2) 1XPBS (negative control) using ab174267 at a 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab174267 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab174267.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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