Overview

  • Product name
  • Description
    Mouse polyclonal to HSPC142
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Cow, Common marmoset, Orangutan
  • Immunogen

    Full length protein, corresponding to amino acids 1-329 of Human HSPC142 (NP_001028721.1).

  • Positive control
    • Hela cells; Lysate of 293T cells transfected with HSPC142.

Properties

Applications

Our Abpromise guarantee covers the use of ab167202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
  • Sequence similarities
    Belongs to the BABAM1 family.
  • Domain
    The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
  • Cellular localization
    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • BABA1_HUMAN antibody
    • babam1 antibody
    • BRCA1 A complex subunit MERIT40 antibody
    • BRISC and BRCA1-A complex member 1 antibody
    • C19orf62 antibody
    • FLJ20571 antibody
    • HSPC142 antibody
    • hypothetical protein LOC29086 antibody
    • Mediator of Rap80 interactions and targeting 40 kDa antibody
    • Mediator of RAP80 interactions and targeting subunit of 40 kDa antibody
    • MERIT 40 antibody
    • MERIT40 antibody
    • NBA1 antibody
    • New component of the BRCA1 A complex antibody
    • New component of the BRCA1-A complex antibody
    • Uncharacterized protein C19orf62 antibody
    see all

Images

  • All lanes : Anti-HSPC142 antibody (ab167202) at 1 µg/ml

    Lane 1 : Lysate of 293T cells transfected with HSPC142
    Lane 2 : Lysate of 293T cells (non-transfected)

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat anti-mouse HRP conjugated antibody at 1/2500 dilution

    Predicted band size: 37 kDa

  • Immunofluorescent analysis of HeLa cells labeling HSPC142 with ab167202 at 10 ug/ml.

References

ab167202 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab167202.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up