Key features and details
- Mouse polyclonal to HSPC142
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HSPC142 antibody
See all HSPC142 primary antibodies
DescriptionMouse polyclonal to HSPC142
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Rat, Cow, Common marmoset, Orangutan
- Hela cells; Lysate of 293T cells transfected with HSPC142.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Constituent: 99% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab167202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionComponent of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
Sequence similaritiesBelongs to the BABAM1 family.
DomainThe VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
Cellular localizationCytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
- Information by UniProt
- BABA1_HUMAN antibody
- babam1 antibody
- BRCA1 A complex subunit MERIT40 antibody
All lanes : Anti-HSPC142 antibody (ab167202) at 1 µg/ml
Lane 1 : Lysate of 293T cells transfected with HSPC142
Lane 2 : Lysate of 293T cells (non-transfected)
Lysates/proteins at 15 µl per lane.
All lanes : Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Predicted band size: 37 kDa
Immunofluorescent analysis of HeLa cells labeling HSPC142 with ab167202 at 10 ug/ml.
ab167202 has not yet been referenced specifically in any publications.