Overview

  • Product name

    Anti-HSPC142 antibody - C-terminal
    See all HSPC142 primary antibodies
  • Description

    Rabbit polyclonal to HSPC142 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human HSPC142 (C terminal). The exact sequence is proprietary.
    Database link: Q9NWV8

  • Positive control

    • WB: HSPC142 transfected HEK-293T whole cell extract.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab227970 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 37 kDa.

Target

  • Function

    Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
  • Sequence similarities

    Belongs to the BABAM1 family.
  • Domain

    The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
  • Cellular localization

    Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
  • Information by UniProt
  • Database links

  • Alternative names

    • BABA1_HUMAN antibody
    • babam1 antibody
    • BRCA1 A complex subunit MERIT40 antibody
    • BRISC and BRCA1-A complex member 1 antibody
    • C19orf62 antibody
    • FLJ20571 antibody
    • HSPC142 antibody
    • hypothetical protein LOC29086 antibody
    • Mediator of Rap80 interactions and targeting 40 kDa antibody
    • Mediator of RAP80 interactions and targeting subunit of 40 kDa antibody
    • MERIT 40 antibody
    • MERIT40 antibody
    • NBA1 antibody
    • New component of the BRCA1 A complex antibody
    • New component of the BRCA1-A complex antibody
    • Uncharacterized protein C19orf62 antibody
    see all

Images

  • All lanes : Anti-HSPC142 antibody - C-terminal (ab227970) at 1/10000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : HSPC142 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 37 kDa



    10% SDS-PAGE gel.

References

ab227970 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab227970.
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