Key features and details
- Rabbit polyclonal to HSPC142 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HSPC142 antibody - C-terminal
See all HSPC142 primary antibodies
DescriptionRabbit polyclonal to HSPC142 - C-terminal
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Rhesus monkey
Recombinant fragment within Human HSPC142 (C terminal). The exact sequence is proprietary.
Database link: Q9NWV8
- WB: HSPC142 transfected HEK-293T whole cell extract.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 37 kDa.|
FunctionComponent of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component.
Sequence similaritiesBelongs to the BABAM1 family.
DomainThe VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes.
Cellular localizationCytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks.
- Information by UniProt
- BABA1_HUMAN antibody
- babam1 antibody
- BRCA1 A complex subunit MERIT40 antibody
All lanes : Anti-HSPC142 antibody - C-terminal (ab227970) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HSPC142 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 37 kDa
10% SDS-PAGE gel.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227970 has not yet been referenced specifically in any publications.