Anti-HSPC150/UBE2T antibody (ab154022)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-HSPC150/UBE2T antibody
See all HSPC150/UBE2T primary antibodies -
Description
Rabbit polyclonal to HSPC150/UBE2T -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human HSPC150/UBE2T aa 135-197 (C terminal).
Database link: Q9NPD8 -
Positive control
- NT2D1 and IMR32 whole cell lysates; Human adrenal gland tumor tissue.
-
General notes
This product was previously labelled as HSPC150
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 10% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab154022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 23 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
-
Database links
- Entrez Gene: 29089 Human
- Omim: 610538 Human
- SwissProt: Q9NPD8 Human
- Unigene: 5199 Human
-
Alternative names
- Cell proliferation inducing gene 50 protein antibody
- Cell proliferation-inducing gene 50 protein antibody
- HSPC150 antibody
see all
Images
-
Western blot - Anti-HSPC150/UBE2T antibody (ab154022)Anti-HSPC150/UBE2T antibody (ab154022) at 1/1000 dilution + NT2D1 whole cell lysate at 30 µg
Predicted band size: 23 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPC150/UBE2T antibody (ab154022)Immunohistochemical analysis of paraffin-embedded Human adrenal gland tumor labeling HSPC150/UBE2T with ab154022 at 1/100 dilution.
References
ab154022 has not yet been referenced specifically in any publications.
