Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
![Western blot - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)](https://www.abcam.com/ps/products/179/ab179802/Images/ab179802-208036-anti-hspc150-antibody-ep9447-western-blot.jpg "Western blot - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)")
Key features and details
- Rabbit monoclonal [EP9447] to HSPC150/UBE2T
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-HSPC150/UBE2T antibody [EP9447]
See all HSPC150/UBE2T primary antibodies -
Description
Rabbit monoclonal [EP9447] to HSPC150/UBE2T -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, Jurkat and 293T cell lysates; HeLa cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP9447 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
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| ICC/IF |
1/50 - 1/100.
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| IP |
1/10 - 1/100.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
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ICC/IF
1/50 - 1/100. |
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IP
1/10 - 1/100. |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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Database links
- Entrez Gene: 29089 Human
- Omim: 610538 Human
- SwissProt: Q9NPD8 Human
- Unigene: 5199 Human
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Alternative names
- Cell proliferation inducing gene 50 protein antibody
- Cell proliferation-inducing gene 50 protein antibody
- HSPC150 antibody
see all
Images
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Western blot - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)All lanes : Anti-HSPC150/UBE2T antibody [EP9447] (ab179802) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa -
![Immunoprecipitation - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)](https://www.abcam.com/ps/products/179/ab179802/Images/ab179802-208038-anti-hspc150-antibody-ep9447-immunoprecipitation.jpg)
Immunoprecipitation - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab179802, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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![Immunocytochemistry/ Immunofluorescence - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)](https://www.abcam.com/ps/products/179/ab179802/Images/ab179802-208037-anti-hspc150-antibody-ep9447-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)Immunofluorescent analysis of HeLa cells labeling HSPC150/UBE2T with ab179802 at 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab179802 has been referenced in 4 publications.
- Zheng YW et al. Role of ubiquitin-conjugating enzyme E2T in the carcinogenesis and progression of pancreatic cancer. Oncol Lett 20:1462-1468 (2020). PubMed: 32724389
- Huang P et al. UBE2T promotes glioblastoma invasion and migration via stabilizing GRP78 and regulating EMT. Aging (Albany NY) 12:10275-10289 (2020). PubMed: 32491994
- Yin H et al. UBE2T promotes radiation resistance in non-small cell lung cancer via inducing epithelial-mesenchymal transition and the ubiquitination-mediated FOXO1 degradation. Cancer Lett 494:121-131 (2020). PubMed: 32590022
- Gong YQ et al. UBE2T silencing suppresses proliferation and induces cell cycle arrest and apoptosis in bladder cancer cells. Oncol Lett 12:4485-4492 (2016). WB . PubMed: 28101210