RabMAb

Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)

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Overview

  • Product name

    Anti-HSPC150/UBE2T antibody [EP9447]
    See all HSPC150/UBE2T primary antibodies

  • Description

    Rabbit monoclonal [EP9447] to HSPC150/UBE2T

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt or IHC-P

  • Species reactivity

    Reacts with: Human

  • Immunogen

    within Human HSPC150/UBE2T aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NPD8

  • Positive control

    • HeLa, Jurkat and 293T cell lysates; HeLa cells.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as HSPC150

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab179802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
ICC/IF 1/50 - 1/100.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.

    • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.

    • Pathway

      Protein modification; protein ubiquitination.

    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Information by UniProt

    • Database links

      • Alternative names

        • Cell proliferation inducing gene 50 protein antibody
        • Cell proliferation-inducing gene 50 protein antibody
        • HSPC150 antibody
        • HSPC150 protein similar to ubiquitin conjugating enzyme antibody
        • PIG50 antibody
        • Ube2t antibody
        • UBE2T_HUMAN antibody
        • Ubiquitin carrier protein T antibody
        • Ubiquitin conjugating enzyme antibody
        • ubiquitin conjugating enzyme E2 T antibody
        • Ubiquitin conjugating enzyme E2T antibody
        • Ubiquitin protein ligase T antibody
        • Ubiquitin-conjugating enzyme E2 T antibody
        • Ubiquitin-protein ligase T antibody
        see all

      Images

      • Western blot - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
        Western blot - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
        All lanes : Anti-HSPC150/UBE2T antibody [EP9447] (ab179802) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : Jurkat cell lysate
        Lane 3 : 293T cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 23 kDa

      • Immunoprecipitation - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
        Immunoprecipitation - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)

        Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab179802, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      • Immunocytochemistry/ Immunofluorescence - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
        Immunocytochemistry/ Immunofluorescence - Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)

        Immunofluorescent analysis of HeLa cells labeling HSPC150/UBE2T with ab179802 at 1/50 dilution.

      References

      This product has been referenced in:

      • Gong YQ  et al. UBE2T silencing suppresses proliferation and induces cell cycle arrest and apoptosis in bladder cancer cells. Oncol Lett 12:4485-4492 (2016). WB . Read more (PubMed: 28101210) »
      See 1 Publication for this product

      Publishing research using ab179802? Please let us know so that we can cite the reference in this datasheet.

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