Anti-HSPC150/UBE2T antibody [EP9447] (ab179802)
Key features and details
- Rabbit monoclonal [EP9447] to HSPC150/UBE2T
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSPC150/UBE2T antibody [EP9447]
See all HSPC150/UBE2T primary antibodies -
Description
Rabbit monoclonal [EP9447] to HSPC150/UBE2T -
Host species
Rabbit -
Tested Applications & Species
Application Species ICC/IF HumanIP HumanWB Human -
Immunogen
within Human HSPC150/UBE2T aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9NPD8 -
Positive control
- HeLa, Jurkat and 293T cell lysates; HeLa cells.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as HSPC150
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EP9447 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab179802 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
---|---|
ICC/IF |
Human
|
IHC-P |
Human
|
IP |
Human
|
WB |
Human
|
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 23 kDa.
|
|
ICC/IF |
1/50 - 1/100.
|
|
IP |
1/10 - 1/100.
|
Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 23 kDa. |
ICC/IF
1/50 - 1/100. |
IP
1/10 - 1/100. |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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Database links
- Entrez Gene: 29089 Human
- Omim: 610538 Human
- SwissProt: Q9NPD8 Human
- Unigene: 5199 Human
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Alternative names
- Cell proliferation inducing gene 50 protein antibody
- Cell proliferation-inducing gene 50 protein antibody
- HSPC150 antibody
see all
Images
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All lanes : Anti-HSPC150/UBE2T antibody [EP9447] (ab179802) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 23 kDa -
Western blot analysis on immunoprecipitation pellet from (1) Jurkat cell lysate or (2) 1X PBS (negative control) using ab179802, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
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Immunofluorescent analysis of HeLa cells labeling HSPC150/UBE2T with ab179802 at 1/50 dilution.
Protocols
Datasheets and documents
References (2)
ab179802 has been referenced in 2 publications.
- Zheng YW et al. Role of ubiquitin-conjugating enzyme E2T in the carcinogenesis and progression of pancreatic cancer. Oncol Lett 20:1462-1468 (2020). PubMed: 32724389
- Gong YQ et al. UBE2T silencing suppresses proliferation and induces cell cycle arrest and apoptosis in bladder cancer cells. Oncol Lett 12:4485-4492 (2016). WB . PubMed: 28101210