Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)

Reviews (1) Submit a question References (1)
Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
  • Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9446] to HSPC150/UBE2T
  • Suitable for: WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-HSPC150/UBE2T antibody [EPR9446]
    See all HSPC150/UBE2T primary antibodies

  • Description

    Rabbit monoclonal [EPR9446] to HSPC150/UBE2T

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human HSPC150/UBE2T aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa and Jurkat cell lysates

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.

  • Pathway

    Protein modification; protein ubiquitination.

  • Sequence similarities

    Belongs to the ubiquitin-conjugating enzyme family.
  • Information by UniProt

  • Database links

    • Alternative names

      • Cell proliferation inducing gene 50 protein antibody
      • Cell proliferation-inducing gene 50 protein antibody
      • HSPC150 antibody
      • HSPC150 protein similar to ubiquitin conjugating enzyme antibody
      • PIG50 antibody
      • Ube2t antibody
      • UBE2T_HUMAN antibody
      • Ubiquitin carrier protein T antibody
      • Ubiquitin conjugating enzyme antibody
      • ubiquitin conjugating enzyme E2 T antibody
      • Ubiquitin conjugating enzyme E2T antibody
      • Ubiquitin protein ligase T antibody
      • Ubiquitin-conjugating enzyme E2 T antibody
      • Ubiquitin-protein ligase T antibody
      see all

    Images

    • Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
      Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
      All lanes : Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 23 kDa
      Observed band size: 23 kDa

    • Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
      Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)

    References (1)

    Publishing research using ab140611? Please let us know so that we can cite the reference in this datasheet.

    ab140611 has been referenced in 1 publication.

    • Hao P  et al. UBE2T promotes proliferation and regulates PI3K/Akt signaling in renal cell carcinoma. Mol Med Rep 20:1212-1220 (2019). PubMed: 31173226

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Western blot abreview for Anti-HSPC150/UBE2T antibody [EPR9446]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Breast cancer)
    Gel Running Conditions
    Reduced Denaturing (4-12)
    Loading amount
    40 µg
    Specification
    Breast cancer
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 29 2021

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com