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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ubiquitin
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RecombinantRabMAb

Recombinant Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)

  • Datasheet
Submit a review Submit a question References (1)

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Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
  • Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9446] to HSPC150/UBE2T
  • Suitable for: WB
  • Reacts with: Human

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Secondary
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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
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Recombinant Human HSPC150/UBE2T protein (ab95318)

View more associated products

Overview

  • Product name

    Anti-HSPC150/UBE2T antibody [EPR9446]
    See all HSPC150/UBE2T primary antibodies
  • Description

    Rabbit monoclonal [EPR9446] to HSPC150/UBE2T
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HSPC150/UBE2T aa 1-100. The exact sequence is proprietary.

  • Positive control

    • HeLa and Jurkat cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as HSPC150

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9446
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ubiquitin E3 Enzymes
    • Other E3 Ligases
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • E2 Ubiquitin Conjugating Enzymes

Associated products

  • Alternative Versions

    • Anti-HSPC150/UBE2T antibody [EPR9446] - BSA and Azide free (ab248901)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Jurkat whole cell lysate (ab7899)
  • Recombinant Protein

    • Recombinant Human HSPC150/UBE2T protein (ab95318)

Applications

Our Abpromise guarantee covers the use of ab140611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.
    • Pathway

      Protein modification; protein ubiquitination.
    • Sequence similarities

      Belongs to the ubiquitin-conjugating enzyme family.
    • Target information above from: UniProt accession Q9NPD8 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 29089 Human
      • Omim: 610538 Human
      • SwissProt: Q9NPD8 Human
      • Unigene: 5199 Human
      • Alternative names

        • Cell proliferation inducing gene 50 protein antibody
        • Cell proliferation-inducing gene 50 protein antibody
        • HSPC150 antibody
        • HSPC150 protein similar to ubiquitin conjugating enzyme antibody
        • PIG50 antibody
        • Ube2t antibody
        • UBE2T_HUMAN antibody
        • Ubiquitin carrier protein T antibody
        • Ubiquitin conjugating enzyme antibody
        • ubiquitin conjugating enzyme E2 T antibody
        • Ubiquitin conjugating enzyme E2T antibody
        • Ubiquitin protein ligase T antibody
        • Ubiquitin-conjugating enzyme E2 T antibody
        • Ubiquitin-protein ligase T antibody
        see all

      Images

      • Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
        Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
        All lanes : Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : Jurkat cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution

        Predicted band size: 23 kDa
        Observed band size: 23 kDa

      • Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
        Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (1)

      Publishing research using ab140611? Please let us know so that we can cite the reference in this datasheet.

      ab140611 has been referenced in 1 publication.

      • Hao P  et al. UBE2T promotes proliferation and regulates PI3K/Akt signaling in renal cell carcinoma. Mol Med Rep 20:1212-1220 (2019). PubMed: 31173226

      Customer reviews and Q&As

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      Please use the links above to contact us or submit feedback about this product.

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