Recombinant Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-HSPC150/UBE2T antibody [EPR9446]
See all HSPC150/UBE2T primary antibodies -
Description
Rabbit monoclonal [EPR9446] to HSPC150/UBE2T -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HSPC150/UBE2T aa 1-100. The exact sequence is proprietary.
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Positive control
- HeLa and Jurkat cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product was previously labelled as HSPC150
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9446 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab140611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro able to catalyze polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the ubiquitin-conjugating enzyme family. - Information by UniProt
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Database links
- Entrez Gene: 29089 Human
- Omim: 610538 Human
- SwissProt: Q9NPD8 Human
- Unigene: 5199 Human
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Alternative names
- Cell proliferation inducing gene 50 protein antibody
- Cell proliferation-inducing gene 50 protein antibody
- HSPC150 antibody
see all
Images
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![Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)](https://www.abcam.com/ps/products/140/ab140611/Images/ab140611-155884-anti-hspc150-antibody-epr9446-western-blot.jpg)
Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611)All lanes : Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 23 kDa
Observed band size: 23 kDa
Protocols
Datasheets and documents
References
This product has been referenced in:
- Hao P et al. UBE2T promotes proliferation and regulates PI3K/Akt signaling in renal cell carcinoma. Mol Med Rep 20:1212-1220 (2019). Read more (PubMed: 31173226) »
