• Product name

    Anti-hSSB1 antibody
  • Description

    Rabbit polyclonal to hSSB1
  • Host species

  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to Human hSSB1 aa 161-211.
    Database link: NP_076973.1

  • Positive control

    • HeLa and 293T whole cell lysates.
  • General notes

    Previously labelled as OBFC2B. 



Our Abpromise guarantee covers the use of ab85752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 33 kDa (predicted molecular weight: 23 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/1000 - 1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Antigen retrieval with Tris-EDTA pH9.0 is recommended for human tissues. Antigen retrieval with citrate buffer pH6.0 is recommended for mouse tissues.


  • Relevance

    Single-stranded DNA (ssDNA)-binding proteins, such as hSSB1, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage. hSSB1 plays an important role in DNA damage response. It influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. It associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins.
  • Cellular localization

    Nuclear. Localizes to nuclear foci following DNA damage.
  • Database links

  • Alternative names

    • 2610036N15Rik antibody
    • hSSB1 antibody
    • LP3587 antibody
    • MGC125161 antibody
    • MGC2731 antibody
    • MGC30353 antibody
    • Nabp2 antibody
    • Nucleic acid binding protein 2 antibody
    • OBFC2B antibody
    • Oligonucleotide/oligosaccharide binding fold containing 2B antibody
    • Oligonucleotide/oligosaccharide binding fold containing protein 2B antibody
    • RGD1308158 antibody
    • Sensor of single strand DNA complex subunit B1 antibody
    • Sensor of ssDNA subunit B1 antibody
    • Single stranded DNA binding protein 1 antibody
    • SOSS complex subunit B1 antibody
    • SSB1 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling hSSB1 with ab85752 at 1/1000 (1µg/ml). Detection: DAB.

  • All lanes : Anti-hSSB1 antibody (ab85752) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Predicted band size: 23 kDa
    Observed band size: 33 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 3 minutes
  • Detection of Human hSSB1 by Western Blot of Immunprecipitate.
    All lanes: ab85752 at 1µg/ml detecting by WB hSSB1 in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1: ab85752 Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 5 seconds.


This product has been referenced in:

  • Chen ZW  et al. FBXL5-mediated degradation of single-stranded DNA-binding protein hSSB1 controls DNA damage response. Nucleic Acids Res 42:11560-9 (2014). Read more (PubMed: 25249620) »
See 1 Publication for this product

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