Overview

  • Product name

    Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]
  • Description

    Mouse monoclonal [10F1] to HSV 1 ICP4 Immediate Early Protein
  • Host species

    Mouse
  • Specificity

    ELISA titer 1/25,600 absorption of 0.10
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Herpes Virus

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    10F1
  • Myeloma

    NS1/1-Ag4-1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/800.
ELISA 1/25600.
ICC/IF 1/25600.

Target

  • Relevance

    This IE protein is a multifunctional protein capable of migrating to the nucleus, binding to DNA, trans-activating other viral genes, and autoregulating its own synthesis. Binding of ICP4 to alpha genes causes repression of their expression. ICP4 also acts as a transcriptional activator of beta and gamma genes.
  • Cellular localization

    Nuclear
  • Alternative names

    • Herpes simplex virus 1 ICP4 antibody
    • HSV RS1 antibody
    • ICP4 antibody
    • Immediate early protein antibody
    • RS1 antibody
    • Transcriptional regulator ICP4 antibody
    see all

Images

  • Lane 1 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/200 dilution
    Lane 2 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/400 dilution
    Lane 3 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/800 dilution
    Lane 4 : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1600 dilution

    All lanes : BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Mouse secondary at 1/5000 dilution
  • All lanes : Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1000 dilution

    Lane 1 : HeLa cells (whole cell lysate) - mock infection, cytosolic fraction
    Lane 2 : HeLa cells (whole cell lysate) - mock infection, membrane fraction
    Lane 3 : HeLa cells (whole cell lysate) - mock infection, nuclear fraction
    Lane 4 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - cytosolic fraction
    Lane 5 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours -membrane fraction
    Lane 6 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - nuclear fraction
    Lane 7 : HeLa cells (whole cell lysate) - mock infected
    Lane 8 : HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated sheep anti-mouse IgG

    Observed band size: 97 kDa
    why is the actual band size different from the predicted?



    As shown, ICP4 is detected mainly in the nuclear fraction of infected cells (lane 6). The presence of ICP4 in the cytosolic fraction (lane 4) probably represents the protein undergoing synthesis.

    See Abreview

  • ab6514 (1/5000) staining HSV1 ICP4 immediate Early Protein in mouse 293T cells transiently transfected with a plasmid expressing HSV ICP4 (green). Cells were formaldehyde-fixed and incubated with the primary antibody for 1 hour. A FITC conjugated rabbit polyclonal antibody was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Merkl PE & Knipe DM Role for a Filamentous Nuclear Assembly of IFI16, DNA, and Host Factors in Restriction of Herpesviral Infection. MBio 10:N/A (2019). Read more (PubMed: 30670617) »
  • Mazzon M  et al. Identification of Broad-Spectrum Antiviral Compounds by Targeting Viral Entry. Viruses 11:N/A (2019). Read more (PubMed: 30791609) »
See all 28 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Specification
HeLa cells
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Feb 11 2009

Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Treatment
Infected with HSV KOS for 16 hours
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Sep 28 2007

Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Vero Cells)
Loading amount
10 µg
Specification
Vero Cells
Treatment
Infected with HSV KOS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Aug 14 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa adherent cells)
Specification
HeLa adherent cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.5 % NP40
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 20 2007

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (293T cells, kidney epithelium)
Specification
293T cells, kidney epithelium
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 27 2006

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