Anti-HSV tag antibody (ab19354)
- Datasheet
- References (2)
- Protocols
Overview
-
Product nameAnti-HSV tag antibody
See all HSV tag primary antibodies -
DescriptionGoat polyclonal to HSV tag
-
Host speciesGoat
-
Tested applicationsSuitable for: Flow Cyt, IP, ELISA, IHC-P, ICC/IFmore details
-
Species reactivityReacts with: Species independent
-
Immunogen
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
-
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
Applications
Our Abpromise guarantee covers the use of ab19354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| IP | Use at an assay dependent concentration. | |
| ELISA | 1/100 - 1/500. | |
| IHC-P | Use at an assay dependent concentration. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
-
RelevanceHSV is a peptide epitope tag, sequence QPELAPEDPED.
-
Alternative names
- HSV epitope tag antibody
- QPELAPEDPED tag antibody
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV tag antibody (ab19354)This image is courtesy of an anonymous Abreviewab19354 staining mouse embryonic brain tissue sections by IHC-P. Sections were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/400 and incubated with the sample for 16 hours at 4°C. A Cy3® conjugated donkey anti-goat antibody was used as the secondary.
-
Immunocytochemistry/ Immunofluorescence - Anti-HSV tag antibody (ab19354)This image is courtesy of an anonymous Abreviewab19354 staining HSV tag in the Human HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 4°C. A FITC-conjugated Donkey anti-Goat polyclonal(1/1000) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Wong FK et al. Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex. PLoS Biol 13:e1002217 (2015). IF . Read more (PubMed: 26252244) »
- Padilla BE et al. Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J Cell Biol 179:981-97 (2007). Flow Cyt, IP . Read more (PubMed: 18039931) »
