Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.2
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab19354 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.
ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|ELISA||1/100 - 1/500.|
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
RelevanceHSV is a peptide epitope tag, sequence QPELAPEDPED.
- HSV epitope tag antibody
- QPELAPEDPED tag antibody
ab19354 staining mouse embryonic brain tissue sections by IHC-P. Sections were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 10% serum for 1 hour at 25°C. The primary antibody was diluted 1/400 and incubated with the sample for 16 hours at 4°C. A Cy3® conjugated donkey anti-goat antibody was used as the secondary.
ab19354 staining HSV tag in the Human HeLa cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Paraformaldehyde, permeabilized with Triton X-100 0.1% and blocked with 5% BSA for 2 hours at 25°C. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 4°C. A FITC-conjugated Donkey anti-Goat polyclonal(1/1000) was used as the secondary antibody.
This product has been referenced in:
- Wong FK et al. Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex. PLoS Biol 13:e1002217 (2015). IF . Read more (PubMed: 26252244) »
- Padilla BE et al. Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J Cell Biol 179:981-97 (2007). Flow Cyt, IP . Read more (PubMed: 18039931) »