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    hsv-tag-antibody-ab19354.pdf

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Tags & Cell Markers Epitope Tags Other Tags HSV Tag
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Anti-HSV tag antibody (ab19354)

  • Datasheet
  • SDS
Reviews (4) Submit a question References (3)

Product price, shipping and contact information

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Key features and details

  • Goat polyclonal to HSV tag
  • Suitable for: Flow Cyt, IP, ELISA
  • Reacts with: Species independent
  • Isotype: IgG

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Overview

  • Product name

    Anti-HSV tag antibody
    See all HSV tag primary antibodies
  • Description

    Goat polyclonal to HSV tag
  • Host species

    Goat
  • Tested applications

    Suitable for: Flow Cyt, IP, ELISAmore details
  • Species reactivity

    Reacts with: Species independent
  • Immunogen

    Synthetic peptide:

    SQPELAPEDPED

    (HSV) conjugated to KLH.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Epitope Tags
    • Other Tags
    • HSV Tag

Associated products

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab19354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (1)
Use at an assay dependent concentration.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP
Use at an assay dependent concentration.
ELISA
1/100 - 1/500.
Notes
Flow Cyt
Use at an assay dependent concentration.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP
Use at an assay dependent concentration.
ELISA
1/100 - 1/500.

Target

  • Relevance

    HSV is a peptide epitope tag, sequence QPELAPEDPED.
  • Alternative names

    • HSV epitope tag antibody
    • QPELAPEDPED tag antibody

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (3)

Publishing research using ab19354? Please let us know so that we can cite the reference in this datasheet.

ab19354 has been referenced in 3 publications.

  • Litke AM  et al. Investigating the Pathogenicity of VSX1 Missense Mutations and Their Association With Corneal Disease. Invest Ophthalmol Vis Sci 59:5824-5835 (2018). PubMed: 30535423
  • Wong FK  et al. Sustained Pax6 Expression Generates Primate-like Basal Radial Glia in Developing Mouse Neocortex. PLoS Biol 13:e1002217 (2015). IF . PubMed: 26252244
  • Padilla BE  et al. Endothelin-converting enzyme-1 regulates endosomal sorting of calcitonin receptor-like receptor and beta-arrestins. J Cell Biol 179:981-97 (2007). Flow Cyt, IP . PubMed: 18039931

Customer reviews and Q&As

Show All Reviews Q&A
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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-HSV tag antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (12% gel)
Sample
Human Purified protein (kidney)
Specification
kidney
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Sep 06 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-HSV tag antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Sep 04 2012

Flow Cytometry abreview for Anti-HSV tag antibody

Excellent
Abreviews
Abreviews
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (Live yeast staining on surface displayed protein)
Specification
Live yeast staining on surface displayed protein
Preparation
Cell harvesting/tissue preparation method: spin down and wash with water
Sample buffer: 1XPBS
Permeabilization
No
Gating Strategy
HSV expression positive
Read More

Dr. Lei Chen

Verified customer

Submitted Aug 23 2012

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-HSV tag antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Embryonic brain)
Specification
Embryonic brain
Fixative
Paraformaldehyde
Antigen retrieval step
None
Permeabilization
Yes - 0.3% Triton X 100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Nov 29 2007

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