Overview

  • Product name
  • Description
    Rabbit polyclonal to HSV1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC - Wholemount, IHC-P, IHC-FoFr, ICC/IF, ELISAmore details
  • Immunogen

    Tissue, cells or virus corresponding to HSV1. Whole rabbit corneal cells, infected with herpes simplex virus type I (strain Mac Intyre), were solubilized with detergent.

  • Positive control
    • IHC-Wm: HSV1 in cornea from pVEGFA-GFP reporter mice at day 3 post-infection with HSV1 strain McKrae. IHC-P: HSV1-infected human tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: 1% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Myeloma
    unknown
  • Isotype
    IgG
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount Use at an assay dependent concentration. PubMed: 21998580
IHC-P Use at an assay dependent concentration.

This antibody may be diluted to a titer of 1:75-1:200 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. Pretreat with citrate buffer pH 6.0.

IHC-FoFr Use at an assay dependent concentration. PubMed: 18042934
ICC/IF Use at an assay dependent concentration. PubMed: 27783096
ELISA Use at an assay dependent concentration. PubMed: 22003387

Target

  • Relevance
    Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low.
  • Alternative names
    • Herpes simplex virus 1 antibody
    • Herpes simplex virus antibody
    • Herpes simplex virus type 1 antibody
    • HSV 1 antibody
    • Human herpesvirus 1 antibody
    • Human herpesvirus type 1 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded HSV1-infected human tissue stained for HSV1 with ab9533 in immunohistochemical analysis.

  • ab9533, staining HSV1 in cornea from pVEGFA-GFP reporter mice at day 3 post-infection with HSV1 strain McKrae.

    Corneas were fixed in 4% paraformaldehyde, and washed with PBS containing 1% Triton X-100 before being stained with primary antibody. A Dylight®549-conjugated donkey anti-rabbit IgG was used as the secondary antibody.

  • Formalin-fixed, paraffin-embedded HSV1-infected human tissue stained for HSV1 with ab9533 in immunohistochemical analysis.

References

This product has been referenced in:
  • Wang Z  et al. NEAT1 modulates herpes simplex virus-1 replication by regulating viral gene transcription. Cell Mol Life Sci 74:1117-1131 (2017). IF . Read more (PubMed: 27783096) »
  • Agelidis AM  et al. Viral Activation of Heparanase Drives Pathogenesis of Herpes Simplex Virus-1. Cell Rep 20:439-450 (2017). Read more (PubMed: 28700944) »
See all 12 Publications for this product

Customer reviews and Q&As

1-7 of 7 Abreviews or Q&A

Question
Answer

I am sorry but the specificity of the antibody has not been studies in detail, I cannot therefore share a great deal of information with you. The antibody has been raised against whole rabbit corneal cells, infected with herpes simplex virus type I. This antibody has been found to react with HSV type 1 specific antigens and with some antigens common for HSV types 1 and 2. The antibody reacts with all the major glycoproteins present in the viral envelope and with at least one core protein as determined by crossed immunoelectrophoresis.

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Lip)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6.0 + .05% Tween-20
Permeabilization
No
Specification
Lip
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde

Dr. Kevin Egan

Verified customer

Submitted Mar 23 2018

Abreviews
Application
Flow Cytometry
Sample
Saccharomyces cerevisiae Cell (live yeast staining)
Specification
live yeast staining
Preparation
Cell harvesting/tissue preparation method: harvest cells and wash with 1XPBS
Sample buffer: 1XPBS
Fixation
no fixation
Permeabilization
No
Gating Strategy
HSV-tag expression

Abcam user community

Verified customer

Submitted Aug 10 2012

Question
Answer

Thank you for your patience in this matter. The product, ab9533 (HSV1 antibody), contains 0.05% sodium azide. Please don't hesitate to contact us again if you have further questions.

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Question
Answer

It is approx 275 ug/ml

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Answer

The concentration of this product is 3 mg/ml.

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Answer

This antibody has not been tested in WB, therefore we can't guarantee results. It will take Abcam approximately 7 days to get the antibody, followed by another 24 hours before it arrives in the US.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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