Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)

Reviews (2)Q&A (3)References (9)

Key features and details

  • Mouse monoclonal [3G9] to HSV1 gC Envelope Protein
  • Suitable for: WB, ELISA, ICC/IF
  • Isotype: IgG2a

Overview

  • Product name

    Anti-HSV1 gC Envelope Protein antibody [3G9]

  • Description

    Mouse monoclonal [3G9] to HSV1 gC Envelope Protein

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details

  • Species reactivity

    Reacts with: Other species

  • Immunogen

    Herpes Virus

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    3G9

  • Myeloma

    NS1/1-Ag4-1

  • Isotype

    IgG2a

  • Light chain type

    kappa

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6509 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/3200.
ELISA 1/102400.
ICC/IF 1/25600.

Target

  • Relevance

    Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell.

  • Alternative names

    • Envelope glycoprotein C antibody
    • GC antibody
    • Glycoprotein C antibody
    • Herpes simplex virus 1 glycoprotein C antibody
    • UL44 antibody
    see all

Images

  • Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)
    Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)Image from Livingston CM et al., PLoS Pathog. 2009 Oct;5(10):e1000619. Epub 2009 Oct 9.Fig 1.; doi:10.1371/journal.ppat.1000619; October 9, 2009, PLoS Pathog 5(10): e1000619.
    Western blot analysis of Vero cells mock-infected or infected with KOS at a multiplicity of infection of 10 for 1, 3, 6 and 9 hours.

    HSV1 gC Envelope Protein was detected using ab6509, at 1/1000 dilution. An HRP-conjugated sheep-anti-mouse IgG (1/20000) was used as the secondary antibody.

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References

Publishing research using ab6509? Please let us know so that we can cite the reference in this datasheet.

ab6509 has been referenced in 9 publications.

  • Saiz-Ros N  et al. Host Vesicle Fusion Protein VAPB Contributes to the Nuclear Egress Stage of Herpes Simplex Virus Type-1 (HSV-1) Replication. Cells 8:N/A (2019). PubMed: 30717447
  • Lu H  et al. Targeting STT3A-oligosaccharyltransferase with NGI-1 causes herpes simplex virus 1 dysfunction. FASEB J 33:6801-6812 (2019). PubMed: 30811219
  • Brun P  et al. Herpes Simplex Virus Type 1 Infects Enteric Neurons and Triggers Gut Dysfunction via Macrophage Recruitment. Front Cell Infect Microbiol 8:74 (2018). WB . PubMed: 29600197
  • Huang CY  et al. Thymidine Kinase-Negative Herpes Simplex Virus 1 Can Efficiently Establish Persistent Infection in Neural Tissues of Nude Mice. J Virol 91:N/A (2017). IHC . PubMed: 27974554
  • Santana S  et al. Oxidative stress enhances neurodegeneration markers induced by herpes simplex virus type 1 infection in human neuroblastoma cells. PLoS One 8:e75842 (2013). PubMed: 24124518
  • Ren Y  et al. Glycoprotein M is important for the efficient incorporation of glycoprotein H-L into herpes simplex virus type 1 particles. J Gen Virol 93:319-29 (2012). PubMed: 22012461
  • Scheper T  et al. The glycoproteins C and G are equivalent target antigens for the determination of herpes simplex virus type 1-specific antibodies. J Virol Methods 166:42-7 (2010). PubMed: 20171247
  • Bastian TW & Rice SA Identification of sequences in herpes simplex virus type 1 ICP22 that influence RNA polymerase II modification and viral late gene expression. J Virol 83:128-39 (2009). WB . PubMed: 18971282
  • Livingston CM  et al. Virus-Induced Chaperone-Enriched (VICE) domains function as nuclear protein quality control centers during HSV-1 infection. PLoS Pathog 5:e1000619 (2009). WB . PubMed: 19816571

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Question

what part of the protein is this antibody directed against?

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Answer

Thank you for your inquiry.  Unfortunately this antibody has not been epitope mapped, so we do not have any further information on where the antibody binds. Please do not hesitate to contact us if you need anything further.  Best of luck in your research.

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Question

Inquiry: Hello I would like to know if these monoclonal mouse antibodies ab0656 and ab6509 directed against HSV-1 gB and HSV-1 gC, Have been used to detect infected neurons in culture. Or If they can work to identify Infected neurons in histological preparations?. And what kind of fixation substance (PFA or Methanol) shoul I use to fix my neuron cells?

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Answer

Thank you very much for your interest in ab6506 and ab6509.

To our knowledge, these products have not been tested inneurons in culture. Therefore, I can offer a discount off a future purchase if you buy one or both of these products now, test it in ICC on neurons and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY OR VALUE OFF OF NEXT ORDER for each antibody you test

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test one or both of these products in ICC on neurons. I will then send a discount code. These codes must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in neurons.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. Each discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product turns out to be unsuitable for this application, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question

Do these antibodies recognize the N-terminus or C-terminus of the protein?

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Answer

I apologize for the delay, but I just heard back for certain that this antibody has not been epitope mapped.  So neither ab6509 nor ab33658 have been mapped. I'm sorry that this information could not be more helpful.  Please contact us with any other questions.

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Western blot abreview for Anti-HSV1 gC Envelope Protein antibody [3G9]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (Vero cells)
Loading amount
10 µg
Specification
Vero cells
Treatment
Infected with HSV1 (KOS strain)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Sep 05 2007

Western blot abreview for Anti-HSV1 gC Envelope Protein antibody [3G9]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa cells)
Loading amount
10 µg
Specification
HeLa cells
Treatment
Infected with HSV KOS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Aug 28 2007

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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