Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)
- Datasheet
- Specific References (7)
- Protocols
Overview
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Product nameAnti-HSV1 gC Envelope Protein antibody [3G9]
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DescriptionMouse monoclonal [3G9] to HSV1 gC Envelope Protein
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Host speciesMouse
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Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHCmore details
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Immunogen
Herpes Virus
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Storage bufferPhosphate buffered saline
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Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone number3G9
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MyelomaNS1/1-Ag4-1
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab6509 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/3200. | |
| ELISA | 1/102400. | |
| ICC/IF | 1/25600. | |
| IHC | Use at an assay dependent concentration. PubMed: 27974554 |
Target
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RelevanceHerpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell.
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Alternative names
- Envelope glycoprotein C antibody
- GC antibody
- Glycoprotein C antibody
see all
Images
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![Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)](https://www.abcam.com/ps/datasheet/images/6/ab6509/HSV1-gC-Envelope-Protein-Primary-antibodies-ab6509-2.jpg)
Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)Image from Livingston CM et al., PLoS Pathog. 2009 Oct;5(10):e1000619. Epub 2009 Oct 9.Fig 1.; doi:10.1371/journal.ppat.1000619; October 9, 2009, PLoS Pathog 5(10): e1000619.Western blot analysis of Vero cells mock-infected or infected with KOS at a multiplicity of infection of 10 for 1, 3, 6 and 9 hours.
HSV1 gC Envelope Protein was detected using ab6509, at 1/1000 dilution. An HRP-conjugated sheep-anti-mouse IgG (1/20000) was used as the secondary antibody.
References
This product has been referenced in:
- Brun P et al. Herpes Simplex Virus Type 1 Infects Enteric Neurons and Triggers Gut Dysfunction via Macrophage Recruitment. Front Cell Infect Microbiol 8:74 (2018). WB . Read more (PubMed: 29600197) »
- Huang CY et al. Thymidine Kinase-Negative Herpes Simplex Virus 1 Can Efficiently Establish Persistent Infection in Neural Tissues of Nude Mice. J Virol 91:N/A (2017). IHC . Read more (PubMed: 27974554) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"