Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506)
Key features and details
- Mouse monoclonal [10B7] to HSV1 + HSV2 gB
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Herpes simplex virus, Human herpesvirus 2
- Isotype: IgG1
Overview
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Product name
Anti-HSV1 + HSV2 gB antibody [10B7] -
Description
Mouse monoclonal [10B7] to HSV1 + HSV2 gB -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Herpes simplex virus, Human herpesvirus 2 -
Immunogen
Tissue, cells or virus corresponding to HSV1 + HSV2 gB.
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Positive control
- African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
10B7 -
Myeloma
NS1/1-Ag4-1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab6506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration.
1/1600 (HSV2) - 1/12800 (HSV1). |
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ELISA |
1/102400.
|
|
ICC/IF |
Use at an assay dependent concentration.
1/6400 (HSV1) - 1/25600 (HSV2). |
Notes |
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WB
Use at an assay dependent concentration. 1/1600 (HSV2) - 1/12800 (HSV1). |
ELISA
1/102400. |
ICC/IF
Use at an assay dependent concentration. 1/6400 (HSV1) - 1/25600 (HSV2). |
Target
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Relevance
There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact. -
Cellular localization
Virus envelope -
Database links
- Entrez Gene: 1487312 Herpes simplex virus
- Entrez Gene: 2703455 Herpes simplex virus
- SwissProt: P08666 Herpes simplex virus
- SwissProt: P10211 Herpes simplex virus
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Alternative names
- Envelope glycoprotein B antibody
- GB antibody
- Glycoprotein B antibody
see all
Images
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Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC). Red: counterstain in the conjugate preparation.
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Lanes 1 & 8 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution
Lane 2 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/3200 dilution
Lane 3 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/6400 dilution
Lane 4 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution
Lane 5 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/200 dilution
Lane 6 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/400 dilution
Lane 7 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/800 dilution
Lanes 1-4 : Vero cells infected with HSV1
Lanes 5-8 : Vero cells infected with HSV2
Performed under reducing conditions.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (32)
ab6506 has been referenced in 32 publications.
- Wang Z et al. Oleanolic Acid Derivative AXX-18 Exerts Antiviral Activity by Inhibiting the Expression of HSV-1 Viral Genes UL8 and UL52. Viruses 14:N/A (2022). PubMed: 35746758
- Shan T et al. Viral UL8 Is Involved in the Antiviral Activity of Oleanolic Acid Against HSV-1 Infection. Front Microbiol 12:689607 (2021). PubMed: 34354687
- Suryawanshi RK et al. mTORC2 confers neuroprotection and potentiates immunity during virus infection. Nat Commun 12:6020 (2021). PubMed: 34650053
- Song X et al. Hsp90 Inhibitors Inhibit the Entry of Herpes Simplex Virus 1 Into Neuron Cells by Regulating Cofilin-Mediated F-Actin Reorganization. Front Microbiol 12:799890 (2021). PubMed: 35082770
- Mertens ME & Knipe DM Herpes Simplex Virus 1 Manipulates Host Cell Antiviral and Proviral DNA Damage Responses. mBio 12:N/A (2021). PubMed: 33563816