Product nameAnti-HSV1 + HSV2 gB antibody [10B7]
DescriptionMouse monoclonal [10B7] to HSV1 + HSV2 gB
Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
- African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab6506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
1/1600 (HSV2) - 1/12800 (HSV1).
|ICC/IF||Use at an assay dependent concentration. 1/6400 (HSV1) - 1/25600 (HSV2).|
RelevanceThere are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact.
Cellular localizationVirus envelope
- Envelope glycoprotein B antibody
- GB antibody
- Glycoprotein B antibody
Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC). Red: counterstain in the conjugate preparation.
Lane 1 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution
Lane 2 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution
Lane 1 : Vero cells infected with HSV1
Lane 2 : Vero cells infected with HSV2
Performed under reducing conditions.
This product has been referenced in:
- Sato E et al. Loss of ATP2A2 Allows Herpes Simplex Virus 1 Infection of a Human Epidermis Model by Disrupting Innate Immunity and Barrier Function. J Invest Dermatol N/A:N/A (2018). Read more (PubMed: 29870688) »
- Zhu Y et al. Ex vivo 2D and 3D HSV-2 infection model using human normal vaginal epithelial cells. Oncotarget 8:15267-15282 (2017). Read more (PubMed: 28146426) »