Overview

  • Product name
    Anti-HSV1 + HSV2 gB antibody [10B7]
  • Description
    Mouse monoclonal [10B7] to HSV1 + HSV2 gB
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Herpes Virus

  • Positive control
    • African Green Monkey Kidney cultured cells(Vero), HSV-1 is strain MacIntyre, HSV-2 is strain G.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    10B7
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration.

1/1600 (HSV2) - 1/12800 (HSV1).

ELISA 1/102400.
ICC/IF Use at an assay dependent concentration. 1/6400 (HSV1) - 1/25600 (HSV2).

Target

  • Relevance
    There are two main types of Herpes Simplex Virus (HSV), 1 and 2. The primary difference between the two types is in where they typically establish latency in the body. HSV1 usually establishes latency in the trigeminal ganglion, a collection of nerve cells near the ear. From there, it tends to recur on the lower lip or face causing cold sores. HSV2 usually resides in the sacral ganglion at the base of the spine. From there, it recurs in the genital area but even this difference is not absolute as in rare cases it can infect any part of the body, including the eyes. Up to 30% of genital herpes are also caused by HSV1. Both HSV1 and 2 are highly contagious through contact.
  • Cellular localization
    Virus envelope
  • Alternative names
    • Envelope glycoprotein B antibody
    • GB antibody
    • Glycoprotein B antibody
    • HSV1 antibody
    • HSV2 antibody
    • UL27 antibody
    see all

Images

  • Green: immunofluorescence staining of HSV1 or HSV2 of Vero (African green monkey) cells ( HSV-1 is strain MacIntyre, HSV-2 is strain G) on slides, using ab6506 (1:100) (secondary anti-mouse-FITC). Red: counterstain in the conjugate preparation.
  • Lane 1 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/12800 dilution
    Lane 2 : Anti-HSV1 + HSV2 gB antibody [10B7] (ab6506) at 1/1600 dilution

    Lane 1 : Vero cells infected with HSV1
    Lane 2 : Vero cells infected with HSV2

    Performed under reducing conditions.

References

This product has been referenced in:
  • Zhu Y  et al. Ex vivo 2D and 3D HSV-2 infection model using human normal vaginal epithelial cells. Oncotarget 8:15267-15282 (2017). Read more (PubMed: 28146426) »
  • Hu Z  et al. Aqueous synthesized quantum dots interfere with the NF-?B pathway and confer anti-tumor, anti-viral and anti-inflammatory effects. Biomaterials 108:187-96 (2016). Read more (PubMed: 27639114) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 10 Abreviews or Q&A

Question
Answer

Thank you for contacting us.

The following article will be helpful for further reading; http://www.ncbi.nlm.nih.gov/pubmed/2475970

We can provide the blocking peptide , which was used for producing this antibody? Could you confirm if you are happy purchasing this and then I will send you the price?

I have checked the WB protocol we used to test this antibody. This protocol is no different than the any general protocol. We recommend using reduced and denaturing conditions.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

I am very pleased to hear you would like to accept our offer and test ab6506 in ICC on neurons. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for this application and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. All orders to Colombia require pre-payment. You can pay by credit card, or our customer service group can send you a proforma invoice which you will need to pay before the goods are shipped - this can be requested when you place an order. Our customer service representatives will also be able to provide you with further delivery information at that time. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab6506 and ab6509.

To our knowledge, these products have not been tested inneurons in culture. Therefore, I can offer a discount off a future purchase if you buy one or both of these products now, test it in ICC on neurons and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY OR VALUE OFF OF NEXT ORDER for each antibody you test

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test one or both of these products in ICC on neurons. I will then send a discount code. These codes must be issued before purchasing so please wait for my reply before ordering.

2. Purchase either by phone, fax, or online (www.abcam.com).

3. Test it in neurons.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. Each discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the product turns out to be unsuitable for this application, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for your enquiry.

The immunogen used to generate the antibody clone [10B7] (ab6506) was the entire Herpes Virus. Unfortunately, the exact epitope recognised by this monoclonal antibody has not been mapped.

To our knowledge this antibody has been shown to have virus neutralizing activity, and thus it is likely that the antibody binds an extracellular domain. We unfortunately do not have data on this available though.

Maybe a search in the literature could help to find more details about this clone. The publication by Reszka and colleagues (PMID 20117270, attached) might be a good starting point.

We aim to provide as much information as possible to customers, so I am sorry that this has not been possible on this occasion. Should you have any further questions, please do not hesitate to contact us.

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Answer

Thank you for confirming these details and for your cooperation.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued an alternative free of charge replacement of ab8232:

Order number: ######

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

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Answer

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or credit note in compensation.

I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this batch. Regrettably, I can suggest you have received a bad vial on this occasion.

Therefore, we would be pleased to provide a replacement of the same antibody, which will still be covered by our guarantee. Alternatively, I fully understand the your concerns and if you prefer an alternative replacement or credit note in this case, I will be pleased to arrange this for you.

We have one other i-HSV1 + HSV2 gB antibody which has been testd in ICC-IF and has ICC-IF image on the datasheet:

HSV1 + HSV2 gB antibody [T111] (ab8232)
https://www.abcam.com/index.html?datasheet=8232 https://www.abcam.com/index.html?
datasheet=8232.

I hope this will be helpful. I look forward to hearing from you with details of how you would like to proceed.

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Answer

Thank you for your inquiry. I heard back from the lab and unfortunately neither ab6506 nor ab33040 have been epitope mapped. We do not know if these antibodies will work in your sELISA since we do not know where the antibodies are binding. I am sorry that this information could not be more helpful. Please contact us with any other questions. Our scientist sent us the following reply to your inquiry. It is as follows: We do not have epitope information. In some cases, you can find a literature reference on the webpage that describes the antibody. This literature may have the epitope information. For pairing experiments, it may be most effective to just try it.

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Answer

Thank you for your enquiry. I have obtained some details on this antibody for you. As it is a virus neutralising antibody, it is highly likely that it binds to the extracellular domain. However, there is no information available on the precise epitope/region of the protein that this antibody is raised against. I hope this helps. Please get back to me if you require more help. With thanks.

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Answer

Thank you for your phone call. I apologize for the shortage that you have received and a replacement is being sent to you free of charge on order# 81816 which you should receive in approximately one week. I did contact the source of this product and they said that sometimes the antibodies slowly lose water under normal storage conditions. You are not short of protein, just volume (water), and they suggested adding 43 ul of deionized water to the vial that you received. However, I am still sending you a replacement vial. Please let me know if you have any additional questions.

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Question
Answer

Hi Joel, Yes it does detect gB from both type 1 and type 2 HSV. Please see our testing data at: HSV gB Mab

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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