Overview

  • Product name
    Anti-HSV1 + HSV2 gD antibody [2C10]
  • Description
    Mouse monoclonal [2C10] to HSV1 + HSV2 gD
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Immunogen

    Herpes Virus

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    2C10
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000.
ELISA 1/102400.
ICC/IF 1/3200 - 1/25600.

1/3,200 (HSV-2) - 1/25,600 (HSV-1).

Target

  • Relevance
    Herpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans. They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body. Under a microscope, HSV- 1 and 2 are virtually identical, sharing approximately 50% of their DNA. Both types infect the body's mucosal surfaces, usually the mouth or genitals, and then establish latency in the nervous system. HSV-1 is commonly associated with herpes outbreaks of the face known as cold sores or fever blisters, whereas HSV-2 is more often associated with genital herpes. Herpes simplex viruses (HSV) use multiple and sequential receptors to enter host cells. HSV glycoprotein D (gD) has been implicated in binding to cellular receptors that facilitate virus penetration into cells. Herpes simplex virus type 1 (HSV-1) glycoprotein D (gD) is an essential component of the entry apparatus that is responsible for viral penetration and subsequent cell-cell spread.
  • Alternative names
    • Envelope glycoprotein D antibody
    • GD antibody
    • Glycoprotein D antibody
    • US6 antibody

Images

  • The WB was completed using the HSV-1 Antigen and the HSV-2 Infected Cell Extract at 10 µg/cm
  • Immunocytochemistry/Immunofluorescence analysis of vero cells infected with HSV1 (left) or HSV2 (right) labelled with ab6507.

References

This product has been referenced in:
  • Aravantinou M  et al. Experimental Oral Herpes Simplex Virus-1 (HSV-1) Co-infection in Simian Immunodeficiency Virus (SIV)-Infected Rhesus Macaques. Front Microbiol 8:2342 (2017). ELISA . Read more (PubMed: 29259582) »
  • Çuburu N  et al. Topical herpes simplex virus 2 (HSV-2) vaccination with human papillomavirus vectors expressing gB/gD ectodomains induces genital-tissue-resident memory CD8+ T cells and reduces genital disease and viral shedding after HSV-2 challenge. J Virol 89:83-96 (2015). WB . Read more (PubMed: 25320297) »
See all 9 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

Could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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Question

Thanks for your reply. Here are the details of the protocol that I followed: Guinea pig fibroblast cells were infected with a recombinant virus with the gene for HSV2gD (from strain 333), HSV1 strain 17Syn+, and HSV2 (strain HG52). The positive controls of HSV were harvested 24 hours post infection; the recombinant virus were incubated until the monolayer was at least 80% infected. The monolayers were harvested using dissociation mix and, after heating at 95 C for 5 minutes, 30 ul of cell lysates were loaded onto a polyacrilimide gel. The gel was transfered to a nitrocellulose membrane (using markers to confirm that the transfer worked). The samples transferred to the membrane included mock infected cells, the backdrop virus, two recombinant virus samples, HSV1 and HSV2. I followed protocol provided in the Western Breeze Kit (anti-mouse) and we have had success with this kit in the past. Again, the dilution used for the HSVgD primary Ab was 1:500. The membrane was incubated for 1 hour with primary Ab the first experimental run. When I repeated the assay, primary Ab incubation was for 3 hours. Both times I performed this Western Blot assay, bands were visible after 1 hour of incubation with the chromogenic substrate only in the lanes containing the recombinant virus samples. In both lanes, a band at 55 KDa and a slightly less intense band just below it were visible on the membrane. No bands were visible in the negative controls, nor in the HSV1 or HSV2 samples. We have a few concerns with these results related to the primary antibody: 1. We used a 1:500 dilution, much more concentration then recommended, yet did not produce a strong signal in the Western Blot. Our lab has had success with this protocol using other antibodies. 2. In repeat Westerns, the postive controls of both HSV1 and HSV2 are not being detected. 3. We have also tried using this Ab in immunofluorescence assays, none which have worked thus far. I appreciate your time and concern with this inquiry, and I believe I supplied all the information I can. Most likely, we are as anxious to understand what is going on as you are, but I cannot invest any more time and due to these time constraints, we are pursuing ordering from another company. Thank you,

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Answer

Thank you for providing these details. When this antibody was characterized in Western blotting with HSV-1 Antigen and the HSV-2 Infected Cell Extract, the antibody worked at dilutions from 1:10,000-1:80,000. As you're seeing a very weak signal using a dilution of 1:500, there definitely seems to be a problem with the antibody that you received. I would be happy to provide you with a free of charge replacement or a refund or credit. Please let me know how you would like to proceed and I look forward to hearing from you.

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Answer

Thank you for your enquiry. I was able to obtain the following information from our source for this antibody about how it was tested in Western blotting. Ab6507 was tested using the HSV-1 Antigen and the HSV-2 Infected Cell Extract at 10 micrograms/cm. A band at 55 kDa was detected, and you can see an image of this Western on the online product datasheet (I just added it). They used a general protocol, the only difference that I saw was that a BCIP/NBT detection kit was used. We always suggest using ECL+ as it's more sensitive. Can you tell me more specifics about your samples and your Western protocol? It'll then be easier to see what may be going on with this antibody. Thank you and I look forward to hearing from you.

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Answer

Thank you for your e-mail and congratulations on your paper. I can certainly arrange a refund on your recent order of ab6507 if the antibody was purchased in the last 90days and if you could provide your order details. We have not received any other complaints about this antibody so it may have been damaged during shipping or storage; as you have tested the new lot with old lots in parallel this indicates that the rest of your experiment is working well and the antibody is damaged. Can I please also suggest you submit an Abreview on the antibodies you have used and in return we will offer you 50 Abpoints per Abreview which can be redeemed on a number of rewards (a further 100 Abpoints will be offered for an image/figure). I look forward to hearing from you regarding your order details,

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Answer

I assume that you are doing frozen section work. 100% acetone for 10 minutes is usually a good fixative for this purpose. Aside, use PBS to dilute the antibody and for washes.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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