Product nameAnti-HSV1 + HSV2 ICP5 Major Capsid Protein antibody [3B6]
DescriptionMouse monoclonal [3B6] to HSV1 + HSV2 ICP5 Major Capsid Protein
Tested applicationsSuitable for: WB, ELISA, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Other species
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab6508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IFA titer: HSV-1 1+ @ 1:3,200; HSV-2 1+ @ 1:3,200
ELISA titer: HSV-1 0.100 @ >1:102,400; HSV-2 0.100 @ >1:102,400
Western Blot Titer: HSV-1 >1:3,200; HSV-2 >1:3,200
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHerpes simplex virus 1 and 2 (HSV-1 and HSV-2) are two species of the herpes virus family, Herpesviridae, which cause infections in humans. They are also called Human Herpes Virus 1 and 2 (HHV-1 and HHV-2) and are neurotropic and neuroinvasive viruses; they enter and hide in the human nervous system, accounting for their durability in the human body.
- Capsid protein VP5 antibody
- Herpes simplex virus 1 major capsid protein antibody
- Herpes simplex virus 2 major capsid protein antibody
ab6508 staining HSV1 + HSV2 ICP5 Major Capsid Protein in Human head and neck cancer tissue and Mouse xenograft tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked for 1 hour at 23°C; antigen retrieval was by heat mediation in a 10mM sodium citrate, pH6. Samples were incubated with primary antibody (1/100 in blocking buffer) for 1 hour at 4°C. A Biotin-conjugated anti-mouse IgG polyclonal (1/250) was used as the secondary antibody.
Immunofluorescence analysis of HSV infected cells, 7 hours (left) or 17 hours (right) post-infection, staining HSV1 + HSV2 ICP5 Major Capsid Protein with ab6508 at 1/200 dilution.
Cells were fixed for 10 min in paraformaldehyde, permeabilized in 0.1% Triton X-100 and blocked in 4% goat serum, 1% bovine serum albumin, before incubating with primary antibody for 30 min at room temperature. An AlexaFluor®647-conjugated goat anti-mouse IgG1 was used as the secondary antibody.
This product has been referenced in:
- Skouboe MK et al. STING agonists enable antiviral cross-talk between human cells and confer protection against genital herpes in mice. PLoS Pathog 14:e1006976 (2018). Read more (PubMed: 29608601) »
- Crameri M et al. MxB is an interferon-induced restriction factor of human herpesviruses. Nat Commun 9:1980 (2018). Read more (PubMed: 29773792) »