Overview

  • Product name

    Anti-HSV1 ICP0 antibody [5H7]
  • Description

    Mouse monoclonal [5H7] to HSV1 ICP0
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Herpes Virus

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    5H7
  • Myeloma

    NS1/1-Ag4-1
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/8000.
ELISA 1/12800.
ICC/IF 1/25600.

Target

  • Relevance

    Herpes simplex virus (HSV) encodes four nuclear phosphoproteins that contribute towards the full productive lytic cycle cascade in newly infected permissive cells. The IE175 (or ICP4) and IE63 (or ICP27) proteins have essential regulatory roles during the lytic cycle. A third, IE110 (or ICP0), appears to play only an accessory role in the presence of VP16, but in the absence of VP16 appears to be critical for initiation of reactivated infections.
  • Cellular localization

    Nuclear
  • Alternative names

    • Alpha 0 protein antibody
    • Herpes siplex virus 1 antibody
    • HHV1 ICP0 antibody
    • HSV1 IE110 antibody
    • Human Herpesvirus 1 antibody
    • IE110 antibody
    • Immediate early nuclear phosphoprotein antibody
    • Immediate early protein IE110 antibody
    • Trans acting transcriptional protein ICP0 antibody
    • VMW110 antibody
    see all

Images

  • ab6513 (1/25600) staining HSV1 ICP0 in human cells. Cells were PFA-fixed, permeabilized in 0.3% TritonX-100/PBS and blocked in 1% serum /30 minutes /25oC. A fluorescently labeled donkey anti-mouse antibody was used as the secondary.

    See Abreview

References

This product has been referenced in:

  • Yan X  et al. miR-138 expression in oral herpes simplex and its effect on ICP0. Exp Ther Med 17:388-392 (2019). Read more (PubMed: 30651809) »
  • Pan D  et al. Herpes Simplex Virus 1 Lytic Infection Blocks MicroRNA (miRNA) Biogenesis at the Stage of Nuclear Export of Pre-miRNAs. MBio 10:N/A (2019). Read more (PubMed: 30755517) »
See all 23 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HSV1 infected or transfected with plasmid)
Specification
HSV1 infected or transfected with plasmid
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% TritonX-100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Oct 12 2007

Answer

According to the datasheet, this antibody has not been tested for immunohistochemistry. I have looked through our catalogue and am very sorry to inform you that we currently do not stock products that suit to your requirement. If you cannot find what you are looking for within the Abcam catalog, I suggest that you click on the link to The World's Antibody Gateway. The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249).

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