Key features and details
- Mouse monoclonal [10A3] to HSV1 ICP8 Major DNA binding protein
- Suitable for: WB, ICC/IF
- Isotype: IgG1
Product nameAnti-HSV1 ICP8 Major DNA binding protein antibody [10A3]
See all HSV1 ICP8 Major DNA binding protein primary antibodies
DescriptionMouse monoclonal [10A3] to HSV1 ICP8 Major DNA binding protein
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Other species
ICP8 purified from U-35-VERO cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Light chain typeunknown
Our Abpromise guarantee covers the use of ab20193 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
RelevanceHerpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. ICP8 is the major DNA binding protein of herpes simplex virus type 1.
- DBP antibody
- Herpes simplex virus type 1 DBP antibody
- HSV1 DBP antibody
ab20193 staining HSV1 ICP8 Major DNA binding protein in Human U2OS by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
ab20193 has been referenced in 9 publications.
- Crameri M et al. MxB is an interferon-induced restriction factor of human herpesviruses. Nat Commun 9:1980 (2018). PubMed: 29773792
- Valledor M et al. Herpes ICP8 protein stimulates homologous recombination in human cells. PLoS One 13:e0200955 (2018). PubMed: 30110337
- Franzoso FD et al. Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1. J Virol 91:N/A (2017). PubMed: 28515305
- Vogel R et al. Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection. J Virol 86:143-55 (2012). PubMed: 22013059
- Luganini A et al. Inhibition of herpes simplex virus type 1 and type 2 infections by peptide-derivatized dendrimers. Antimicrob Agents Chemother 55:3231-9 (2011). PubMed: 21576438
- Glauser DL et al. Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities. J Virol 84:3808-24 (2010). WB, ICC/IF . PubMed: 20106923
- Souki SK et al. Arginine methylation of the ICP27 RGG box regulates ICP27 export and is required for efficient herpes simplex virus 1 replication. J Virol 83:5309-20 (2009). WB . PubMed: 19321610
- de Oliveira AP et al. Live visualization of herpes simplex virus type 1 compartment dynamics. J Virol 82:4974-90 (2008). WB . PubMed: 18337577
- Glauser DL et al. Live covisualization of competing adeno-associated virus and herpes simplex virus type 1 DNA replication: molecular mechanisms of interaction. J Virol 81:4732-43 (2007). WB . PubMed: 17314170