Overview

  • Product name
    Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3]
    See all HSV1 ICP8 Major DNA binding protein primary antibodies
  • Description
    Mouse monoclonal [10A3] to HSV1 ICP8 Major DNA binding protein
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with Herpes Simplex Virus.
  • Immunogen

    ICP8 purified from U-35-VERO cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein A/G purified
  • Clonality
    Monoclonal
  • Clone number
    10A3
  • Myeloma
    Sp2
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • Relevance
    Herpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. ICP8 is the major DNA binding protein of herpes simplex virus type 1.
  • Alternative names
    • DBP antibody
    • Herpes simplex virus type 1 DBP antibody
    • HSV1 DBP antibody
    • ICP8 antibody
    • Infected cell protein 8 antibody
    • Major DNA binding protein antibody
    • UL29 antibody
    see all

Images

  • ab20193 staining HSV1 ICP8 Major DNA binding protein in Human U2OS by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Crameri M  et al. MxB is an interferon-induced restriction factor of human herpesviruses. Nat Commun 9:1980 (2018). Read more (PubMed: 29773792) »
  • Franzoso FD  et al. Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1. J Virol 91:N/A (2017). Read more (PubMed: 28515305) »
See all 8 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 22°C
Sample
Human Cell (U2OS)
Specification
U2OS
Permeabilization
Yes - 0.5% tritonx-100
Fixative
Paraformaldehyde

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Verified customer

Submitted Nov 27 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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