Product nameAnti-HSV1 ICP8 Major DNA binding protein antibody [11E2]
See all HSV1 ICP8 Major DNA binding protein primary antibodies
DescriptionMouse monoclonal [11E2] to HSV1 ICP8 Major DNA binding protein
Tested applicationsSuitable for: IP, WB, ICC/IFmore details
Species reactivityReacts with HSV1. Not yet tested in other species.
Full length native protein (purified from U-35-VERO cells) (HSV).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A/G purified
Our Abpromise guarantee covers the use of ab20194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 129 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHerpes simplex type 1 (HSV-1) belongs to a family that includes HSV-2, Epstein-Barr virus (EBV) and Varicella zoster (chicken pox) virus amongst others. HSV-1 and HSV-2 are extremely difficult to distinguish from each other. Members of this family have a characteristic virion structure. The double stranded DNA genome is contained within an icosahedral capsid embedded in a proteinaceous layer (tegument) and surrounded by a lipid envelope, derived from the nuclear membrane of the last host, which is decorated with virus-specific glycoproteins spikes. These viruses are capable of entering a latent phase where the host shows no visible sign of infection and levels of infectious agent become very low. During the latent phase the viral DNA is integrated into the genome of the host cell. ICP8 is the major DNA binding protein of herpes simplex virus type 1.
- DBP antibody
- Herpes simplex virus type 1 DBP antibody
- HSV1 DBP antibody
ab20194 staining HSV1 ICP8 Major DNA binding protein in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Immunofluorescence analysis of cells infected with HSV1, staining HSV1 ICP8 Major DNA binding protein (purple) with ab20194, 7 (left) or 17 (right) hours after infection.
Cells were permeabilized in 0.1% Triton X-100 in PBS for 5 min at room temperature before blocking with blocking buffer (4% goat serum, 1% BSA in PBS-Tween [0.05%]) for 30 min at room temperature. Samples were incubated with primary antibody (1/1000) and a fluorescence conjugated anti-mouse IgG was used to detect staining.
Immunofluorescence analysis of HeLa cells transfected with pTF3 and pRF, staining HSV1 ICP8 Major DNA binding protein with ab20194.
Cells were fixed in paraformaldehyde for 10 min and then permeabilized with 0.5% Triton X-100 for 30 min. The cells were blocked with 4% BSA + 0.2% Tween for 30 min before incubation for 1 hour at RT with primary antibody (1/200 diluted in PBS-T). An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/2000) was used as the secondary antibody.
This product has been referenced in:
- Döhner K et al. Importin a1 is required for nuclear import of herpes simplex virus proteins and capsid assembly in fibroblasts and neurons. PLoS Pathog 14:e1006823 (2018). Read more (PubMed: 29304174) »
- Dembowski JA et al. Replication-Coupled Recruitment of Viral and Cellular Factors to Herpes Simplex Virus Type 1 Replication Forks for the Maintenance and Expression of Viral Genomes. PLoS Pathog 13:e1006166 (2017). Read more (PubMed: 28095497) »