Anti-HSV2 ICP4 antibody (ab96431)
Key features and details
- Rabbit polyclonal to HSV2 ICP4
- Suitable for: WB, ELISA
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-HSV2 ICP4 antibody
See all HSV2 ICP4 primary antibodies -
Description
Rabbit polyclonal to HSV2 ICP4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Recombinant fragment
Predicted to work with: Other species -
Immunogen
A synthetic peptide corresponding to N terminal residues of Human HSV2 ICP4
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purity is >90% -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96431 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Predicted molecular weight: 135 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
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ELISA |
1/2000 - 1/5000.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 135 kDa. for 2 hours. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein. |
ELISA
1/2000 - 1/5000. |
Target
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Relevance
ICP4 (Trans-acting transcriptional protein ICP4) is a transcriptional transactivator that binds with high affinity to the sequence 5'-ATCGTC-3'. ICP4 may interact with and recruit specific components of the general transcription machinery to viral promoters and stabilize their formation for transcription initiation. ICP4 negatively regulates its own transcription. This immediate early (EI) protein may be necessary in virion for viral pathogenesis. ICP4 is a homodimer and interacts with transcriptional regulator ICP27; this interaction is required for proper incorporation of ICP4 into virions. The long stretch of Ser is a major site of phosphorylation. Only the phosphorylated forms are capable of interacting with beta or gamma genes. ICP4 belongs to the herpesviridae ICP4/IE140/IE180 family. -
Alternative names
- Alpha 4 protein antibody
- Alpha4 protein antibody
- ICP4 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab96431 has been referenced in 3 publications.
- Sureram S et al. The Peptide A-3302-B Isolated from a Marine Bacterium Micromonospora sp. Inhibits HSV-2 Infection by Preventing the Viral Egress from Host Cells. Int J Mol Sci 23:N/A (2022). PubMed: 35055133
- Zhang M et al. Herpes Simplex Virus Type 2 Infection-Induced Expression of CXCR3 Ligands Promotes CD4+ T Cell Migration and Is Regulated by the Viral Immediate-Early Protein ICP4. Front Immunol 9:2932 (2018). PubMed: 30619292
- Stanley JA et al. A fetal whole ovarian culture model for the evaluation of CrVI-induced developmental toxicity during germ cell nest breakdown. Toxicol Appl Pharmacol 289:58-69 (2015). PubMed: 26348139