Anti-HTF9C/TRMT2A antibody (ab121126)
Key features and details
- Rabbit polyclonal to HTF9C/TRMT2A
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HTF9C/TRMT2A antibody
See all HTF9C/TRMT2A primary antibodies -
Description
Rabbit polyclonal to HTF9C/TRMT2A -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human HTF9C/TRMT2A aa 462-621.
Database link: Q8IZ69 -
Positive control
- WB:RT4, EFO-21, HEK293 and A431 cell lysates; IHC-P: Human oral mucosa tissue; ICC/IF: U-251MG cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121126 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69 kDa.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 69 kDa. |
Target
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Function
May be involved in nucleic acid metabolism and/or modifications. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. - Information by UniProt
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Database links
- Entrez Gene: 27037 Human
- Omim: 611151 Human
- SwissProt: Q8IZ69 Human
- Unigene: 713579 Human
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Alternative names
- HpaII tiny fragments locus 9c protein antibody
- HTF9C antibody
- TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
see all
Images
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Anti-HTF9C/TRMT2A antibody (ab121126) at 0.4 µg/ml + HEK-293 cell lysate
Predicted band size: 69 kDa -
Immunohistochemical analysis of paraffin-embedded human oral mucosa staining HTF9C/TRMT2A with ab121126 at 1/50 dilution.
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Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121126 1-4 µg/ml. Cells treated with PFA/Triton X-100.
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All lanes : Anti-HTF9C/TRMT2A antibody (ab121126) at 1/250 dilution
Lane 1 : RT4 cell lysate
Lane 2 : EFO-21 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Human Liver lysate
Lane 5 : Human Tonsil lysate
Developed using the ECL technique.
Predicted band size: 69 kDa
10-20% SDS-PAGE.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121126 has not yet been referenced specifically in any publications.