Key features and details
- Mouse monoclonal [1G8] to HTF9C/TRMT2A
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-HTF9C/TRMT2A antibody [1G8]
See all HTF9C/TRMT2A primary antibodies
DescriptionMouse monoclonal [1G8] to HTF9C/TRMT2A
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human HTF9C/TRMT2A. (NP_073564). Produced in HEK293T cells.
Database link: Q8IZ69
- WB: HeLa, MOLT-4, pCMV6-ENTRY HTF9C/TRMT2A-transfected HEK-293T whole cell lysates. IHC-P: Human testis tissue.
This product was previously labelled as HTF9C
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
PurityProtein A/G purified
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab230922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||1/2000. Predicted molecular weight: 69 kDa.|
FunctionMay be involved in nucleic acid metabolism and/or modifications.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- HpaII tiny fragments locus 9c protein antibody
- HTF9C antibody
- TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
All lanes : Anti-HTF9C/TRMT2A antibody [1G8] (ab230922) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRMT2A knockout HeLa cell lysate
Lane 3 : MOLT-4 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Lanes 1-3: Merged signal (red and green). Green - ab230922 observed at 75 kDa. Red - loading control ab181602 observed at 36 kDa.
ab230922 Anti-HTF9C/TRMT2A antibody [1G8] was shown to specifically react with HTF9C/TRMT2A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265160 (knockout cell lysate ab258253) was used. Wild-type and HTF9C/TRMT2A knockout samples were subjected to SDS-PAGE. ab230922 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2. 5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Formalin-fixed, paraffin-embedded human testis tissue stained for HTF9C/TRMT2A using ab230922 at 10 μg/ml in immunohistochemical analysis.
All lanes : Anti-HTF9C/TRMT2A antibody [1G8] (ab230922) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY HTF9C/TRMT2A-transfected HEK-293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230922 has not yet been referenced specifically in any publications.