Anti-HTF9C/TRMT2A antibody [1G8] (ab230922)
Key features and details
- Mouse monoclonal [1G8] to HTF9C/TRMT2A
- Suitable for: IHC-P, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Overview
-
Product name
Anti-HTF9C/TRMT2A antibody [1G8]
See all HTF9C/TRMT2A primary antibodies -
Description
Mouse monoclonal [1G8] to HTF9C/TRMT2A -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human HTF9C/TRMT2A. (NP_073564). Produced in HEK293T cells.
Database link: Q8IZ69 -
Positive control
- WB: HeLa, MOLT-4, pCMV6-ENTRY HTF9C/TRMT2A-transfected HEK-293T whole cell lysates. IHC-P: Human testis tissue.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
1G8 -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
WB |
1/2000. Predicted molecular weight: 69 kDa.
|
Notes |
---|
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/2000. Predicted molecular weight: 69 kDa. |
Target
-
Function
May be involved in nucleic acid metabolism and/or modifications. -
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain. - Information by UniProt
-
Database links
- Entrez Gene: 27037 Human
- Omim: 611151 Human
- SwissProt: Q8IZ69 Human
- Unigene: 713579 Human
-
Alternative names
- HpaII tiny fragments locus 9c protein antibody
- HTF9C antibody
- TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
see all
Images
-
All lanes : Anti-HTF9C/TRMT2A antibody [1G8] (ab230922) at 1/500 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TRMT2A knockout HeLa cell lysate
Lane 3 : MOLT-4 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab230922 observed at 75 kDa. Red - loading control ab181602 observed at 36 kDa.
ab230922 Anti-HTF9C/TRMT2A antibody [1G8] was shown to specifically react with HTF9C/TRMT2A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265160 (knockout cell lysate ab258253) was used. Wild-type and HTF9C/TRMT2A knockout samples were subjected to SDS-PAGE. ab230922 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated at room temperature for 2. 5 hours at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
Formalin-fixed, paraffin-embedded human testis tissue stained for HTF9C/TRMT2A using ab230922 at 10 μg/ml in immunohistochemical analysis.
-
All lanes : Anti-HTF9C/TRMT2A antibody [1G8] (ab230922) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : pCMV6-ENTRY HTF9C/TRMT2A-transfected HEK-293T whole cell lysate
Lysates/proteins at 5 µg per lane.
Developed using the ECL technique.
Predicted band size: 69 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab230922 has not yet been referenced specifically in any publications.