Overview

  • Product name

    Anti-HTF9C/TRMT2A antibody
    See all HTF9C/TRMT2A primary antibodies
  • Description

    Rabbit polyclonal to HTF9C/TRMT2A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human HTF9C/TRMT2A aa 462-621.
    Database link: Q8IZ69

  • Positive control

    • WB:RT4, EFO-21, HEK293 and A431 cell lysates; IHC-P: Human oral mucosa tissue; ICC/IF: U-251MG cells.
  • General notes

     This product was previously labelled as HTF9C

     

Properties

Applications

Our Abpromise guarantee covers the use of ab121126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500. Predicted molecular weight: 69 kDa.

Target

  • Function

    May be involved in nucleic acid metabolism and/or modifications.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • HpaII tiny fragments locus 9c protein antibody
    • HTF9C antibody
    • TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
    • TRM2 tRNA methyltransferase 2 homolog A antibody
    • TRM2A_HUMAN antibody
    • TRMT2A antibody
    • tRNA (uracil-5-)-methyltransferase homolog A antibody
    • tRNA methyltransferase 2 homolog A antibody
    • tRNA methyltransferase 2, S. cerevisiae, homolog of, A antibody
    see all

Images

  • Anti-HTF9C/TRMT2A antibody (ab121126) at 0.4 µg/ml + HEK-293 cell lysate

    Predicted band size: 69 kDa

  • Immunohistochemical analysis of paraffin-embedded human oral mucosa staining HTF9C/TRMT2A with ab121126 at 1/50 dilution.

  • Immunofluorescent staining of Human cell line U-251MG shows positivity in nucleus and cytoplasm. Recommended concentration of ab121126 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-HTF9C/TRMT2A antibody (ab121126) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : EFO-21 cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate

    Developed using the ECL technique.

    Predicted band size: 69 kDa



    10-20% SDS-PAGE.

References

ab121126 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab121126.
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