Key features and details
- Rabbit polyclonal to HTF9C/TRMT2A - C-terminal
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HTF9C/TRMT2A antibody - C-terminal
See all HTF9C/TRMT2A primary antibodies
DescriptionRabbit polyclonal to HTF9C/TRMT2A - C-terminal
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment within Human HTF9C/TRMT2A (C terminal). The exact sequence is proprietary.
Database link: Q8IZ69
- WB: HEK-293T, A431 and HeLa whole cell extracts. IHC-P: Human lung cancer tissue. ICC/IF: HeLa cells.
This product was previously labelled as HTF9C
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229733 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000.|
|WB||1/1000 - 1/10000. Predicted molecular weight: 69 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay be involved in nucleic acid metabolism and/or modifications.
Sequence similaritiesContains 1 RRM (RNA recognition motif) domain.
- Information by UniProt
- HpaII tiny fragments locus 9c protein antibody
- HTF9C antibody
- TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
All lanes : Anti-HTF9C/TRMT2A antibody - C-terminal (ab229733) at 1/3500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 69 kDa
7.5% SDS-PAGE gel.
Paraffin-embedded human lung cancer tissue stained for HTF9C/TRMT2A using ab229733 at 1/500 dilution in immunohistochemical analysis.
4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HTF9C/TRMT2A (green) using ab229733 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229733 has not yet been referenced specifically in any publications.