Overview

  • Product name

    Anti-HTF9C/TRMT2A antibody - C-terminal
    See all HTF9C/TRMT2A primary antibodies
  • Description

    Rabbit polyclonal to HTF9C/TRMT2A - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human HTF9C/TRMT2A (C terminal). The exact sequence is proprietary.
    Database link: Q8IZ69

  • Positive control

    • WB: HEK-293T, A431 and HeLa whole cell extracts. IHC-P: Human lung cancer tissue. ICC/IF: HeLa cells.
  • General notes

     This product was previously labelled as HTF9C

     

Properties

Applications

Our Abpromise guarantee covers the use of ab229733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/1000 - 1/10000. Predicted molecular weight: 69 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May be involved in nucleic acid metabolism and/or modifications.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • HpaII tiny fragments locus 9c protein antibody
    • HTF9C antibody
    • TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae) antibody
    • TRM2 tRNA methyltransferase 2 homolog A antibody
    • TRM2A_HUMAN antibody
    • TRMT2A antibody
    • tRNA (uracil-5-)-methyltransferase homolog A antibody
    • tRNA methyltransferase 2 homolog A antibody
    • tRNA methyltransferase 2, S. cerevisiae, homolog of, A antibody
    see all

Images

  • All lanes : Anti-HTF9C/TRMT2A antibody - C-terminal (ab229733) at 1/3500 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 69 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded human lung cancer tissue stained for HTF9C/TRMT2A using ab229733 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HTF9C/TRMT2A (green) using ab229733 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab229733 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab229733.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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