Overview

  • Product name

    Anti-HTLV I p24 antibody [46/3.24.4]
  • Description

    Mouse monoclonal [46/3.24.4] to HTLV I p24
  • Host species

    Mouse
  • Specificity

    Reacts with Human T-Lymphotropic Virus Type I (HTLV I) core p24. Cross reactivity with p24 from HTLV II has been observed.
  • Tested applications

    Suitable for: ICC/IF, Radioimmunoprecipitation, WB, ELISAmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    HTVL viral lysate

  • General notes


    The antibody is used to detect HTLV I core protein in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. The antibody is also valuable for the affinity isolation of HTLV I core protein.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified from serum-free culture supernatant.
  • Primary antibody notes

    The antibody is used to detect HTLV I core protein in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. The antibody is also valuable for the affinity isolation of HTLV I core protein.
  • Clonality

    Monoclonal
  • Clone number

    46/3.24.4
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 19008946
Radioimmunoprecipitation Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Human T-lymphotropic virus (HTLV) belongs to the family Retroviridae, genus Deltaretrovirus. It is a human, single-stranded RNA retrovirus that causes T-cell leukemia and T-cell lymphoma in adults and may also be involved in certain demyelinating diseases. Adult T-lymphotropic virus (ATLV) is a strain of this disease that effect primarily adults. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localization

    Core protein
  • Database links

    • Alternative names

      • CA antibody
      • Capsid protein p24 antibody
      • p24 antibody

    Images

    • Immunofluorescence analysis of HTLV-1-infected T cells, staining HTLV I p24 with ab9081.

      Cells were fixed with paraformaldehyde before incubating with 10% normal goat serum and 0.05% saponin for 30 minutes. Cells were incubated with primary antibody and a fluoroscein-conjugated anti-mouse IgG was used as the secondary antibody.
    • Immunofluorescence analysis of HeLa cells transiently transfected with pEYFP-N3-HTLV-1 Gag, staining HTLV I p24 with ab9081.

      Cells were fixed with paraformaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% normal donkey serum for 30 minutes. Samples were incubated with primary antibody (1/150 in blocking solution) for 2 hours at room temperature. An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/250) was used as the secondary antibody.

    References

    This product has been referenced in:

    • Martin-Latil S  et al. Transcytosis of HTLV-1 across a tight human epithelial barrier and infection of subepithelial dendritic cells. Blood 120:572-80 (2012). Read more (PubMed: 22589473) »
    • Grigsby IF  et al. Biophysical analysis of HTLV-1 particles reveals novel insights into particle morphology and Gag stochiometry. Retrovirology 7:75 (2010). WB, ICC/IF . Read more (PubMed: 20854688) »
    See all 3 Publications for this product

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