Key features and details
- Rabbit polyclonal to htrA1
- Suitable for: WB
- Reacts with: Escherichia coli
- Isotype: IgG
Product nameAnti-htrA1 antibody
See all htrA1 primary antibodies
DescriptionRabbit polyclonal to htrA1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Escherichia coli
Recombinant fragment (His-tag) corresponding to Escherichia coli htrA1 aa 304-442. Expressed in E.coli. N-terminal tag.
MGHHHHHHSGS-KAMKVDAQRGAFVSQVLPNSSAAKAGI KAGDVITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLEL QQSSQNQVDSSTIFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKG DVIIGANQQAVK
- WB: Recombinant E. coli htrA1 protein. E.coli lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesFollowed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab231195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.2 - 2 µg/ml.|
FunctionProtease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
Tissue specificityExpressed in a variety of tissues, with strongest expression in placenta.
Involvement in diseaseVariations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
- ARMD7 antibody
- CARASIL antibody
- High-temperature requirement A serine peptidase 1 antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231195 has not yet been referenced specifically in any publications.