Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab262852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 - 4 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionProtease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
Tissue specificityExpressed in a variety of tissues, with strongest expression in placenta.
Involvement in diseaseVariations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
- ARMD7 antibody
- CARASIL antibody
- High-temperature requirement A serine peptidase 1 antibody
PFA-fixed, Triton X-100 permeabilized human LHCN-M2 cells stained for htrA1 (green) using ab262852 at 4 µg/ml in ICC/IF.
Paraffin-embedded Human cervix uterine tissue stained for htrA1 using ab262852 at 1/20 dilution in immunohistochemical analysis. High expression in uterine is observed.
Paraffin-embedded Human testis tissue stained for htrA1 using ab262852 at 1/20 dilution in immunohistochemical analysis. Shows low expression as expected.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab262852 has not yet been referenced specifically in any publications.