Product nameAnti-htrA1 antibody
See all htrA1 primary antibodies
DescriptionRabbit polyclonal to htrA1
Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Rat
Synthetic peptide corresponding to Human htrA1 aa 381-412 conjugated to Keyhole Limpet Haemocyanin (KLH).
Database link: 5654
- Human placenta tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Our Abpromise guarantee covers the use of ab38610 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/500. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionProtease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
Tissue specificityExpressed in a variety of tissues, with strongest expression in placenta.
Involvement in diseaseVariations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
- ARMD7 antibody
- CARASIL antibody
- High-temperature requirement A serine peptidase 1 antibody
Anti-htrA1 antibody (ab38610) at 1/1000 dilution + Human placenta tissue lysate at 35 µg/ml
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling htrA1 with ab38610. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
This product has been referenced in:
- Cheng H et al. HtrA1 suppresses the growth of pancreatic cancer cells by modulating Notch-1 expression. Braz J Med Biol Res 52:e7718 (2018). Read more (PubMed: 30484491) »
- Altobelli E et al. Low HtrA1 expression in patients with long-standing ulcerative colitis and colorectal cancer. Oncol Rep 38:418-426 (2017). Read more (PubMed: 28586045) »