• Product name
  • Description
    Rabbit polyclonal to htrA1
  • Host species
  • Specificity
    Targets N terminal of htrA1.
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human htrA1 aa 116-147 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q92743
    (Peptide available as ab112516)

  • Positive control
    • WB: Mouse brain tissue lysate. IHC-P: Human kidney tissue. Flow Cyt: HeLa and HepG2 cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab38611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 5 µg/ml.
WB 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-P 1/10 - 1/50.


  • Function
    Protease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
  • Tissue specificity
    Expressed in a variety of tissues, with strongest expression in placenta.
  • Involvement in disease
    Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
    Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
  • Sequence similarities
    Belongs to the peptidase S1B family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • ARMD7 antibody
    • CARASIL antibody
    • High-temperature requirement A serine peptidase 1 antibody
    • HtrA antibody
    • HtrA serine peptidase 1 antibody
    • HTRA1 antibody
    • HTRA1_HUMAN antibody
    • IGFBP5 protease antibody
    • L56 antibody
    • ORF480 antibody
    • Protease serine 11 (IGF binding) antibody
    • protease serine 11 antibody
    • PRSS11 antibody
    • Serine protease 11 antibody
    • Serine protease HTRA1 antibody
    • Serine protease HTRA1 precursor antibody
    see all


  • Anti-htrA1 antibody (ab38611) at 1/2000 dilution + Mouse brain lysate at 20 µg

    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size: 51 kDa

    Blocking buffer 5% NFDM/TBST

    Diluting buffer 5% NFDM/TBST

  • Anti-htrA1 antibody (ab38611) + Mouse brain tissue lysate.

    Predicted band size: 51 kDa
    Observed band size: 51 kDa
    Additional bands at: 28 kDa, 35 kDa. We are unsure as to the identity of these extra bands.

  • ab38611 staining htrA1 in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.2% Triton X-100 in PBS and blocked with 5% milk for 30 minutes at room temperature; antigen retrieval was by heat mediation in 10mM sodium citrate + 0.05% Tween. Samples were incubated with primary antibody (1/50 in PBS) for 16 hours at 4°C. An Alexa Fluor® 594-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling htrA1 with ab38611. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling htrA1 (green) with ab38611. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody. Nuclei were stained with DAPI (blue).

  • ICC/IF image of ab38611 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38611, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
  • Hopper N  et al. Increased sclerostin associated with stress fracture of the third metacarpal bone in the Thoroughbred racehorse. Bone Joint Res 7:94-102 (2018). Read more (PubMed: 29363519) »
  • Sheffield ID  et al. Osteoarthritis-Like Changes in Bardet-Biedl Syndrome Mutant Ciliopathy Mice (Bbs1M390R/M390R): Evidence for a Role of Primary Cilia in Cartilage Homeostasis and Regulation of Inflammation. Front Physiol 9:708 (2018). Read more (PubMed: 29971011) »
See all 13 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris pH 9.0
Human Tissue sections (human pancreas carcinoma)
human pancreas carcinoma
Yes - 0.2% Triton X-100 and PBS

Mrs. Maria Cecilia Ybanez

Verified customer

Submitted Mar 17 2014

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Sodium Citrate, 0.05% Tween
Human Tissue sections (human liver)
human liver
Yes - 0.2% Triton X-100 and PBS

Dr. Steffen Rickelt

Verified customer

Submitted Dec 24 2013


This product has been tested under reducing and denaturing conditions.

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Thank you for your reply. Blocking longer may help to further reduce the additional background bands observed, however as shown in the image on our datasheet, this antibody is known to detect some non-specific bands in WB. Could you please provide some information about how the nuclear lysates were prepared? Since htrA1 is a secreted protein, we would expect the band to be weaker in the nuclear fraction. It would also help us to interpret the results if you could please provide additional information about the species and sample type being tested. I would be happy to assist you further if you have any additional questions or concerns.

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Thank you for contacting us. I am sorry that your customer is obtaining high background using ab38611 in WB. What species did the samples come from and what tissue type or cell line was used? Which lanes correspond to the cytoplasmic and nuclear extracts in the image? The customer may be able to reduce his or her background by blocking longer (2-3 hours or up to overnight at 4C). The specificity may also be increased by using less primary and secondary antibody (try a 1:1000 dilution for the primary and 1:5000 for the secondary). I hope this will help to improve his or her results, if not I would be happy to offer a free of charge replacement or a credit if the customer has purchased the product in the past six months and is using it on a tested species. If you could please provide the original order number and let me know how the customer would like to proceed, I would be happy to assist you further.

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