Overview

  • Product name

    Anti-htrA1 antibody [EPR14605(2)]
    See all htrA1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14605(2)] to htrA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IP, Flow Cyt, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human htrA1 aa 350-450. The exact sequence is proprietary.
    Database link: Q92743

  • Positive control

    • WB: HepG2 and MCF7 cell lysates; Human fetal liver tissue lysate. ICC/IF: MCF7 cells. Flow Cyt: HepG2 cells. IP: HepG2 whole cell extract.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14605(2)
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1200.
IP 1/40.
Flow Cyt 1/150.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

WB 1/1000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).

Target

  • Function

    Protease that regulate the availability of nsulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Represses signaling by TGF-beta family members.
  • Tissue specificity

    Expressed in a variety of tissues, with strongest expression in placenta.
  • Involvement in disease

    Variations in the promoter region of HTRA1 are the cause of susceptibility to age-related macular degeneration type 7 (ARMD7) [MIM:610149]. ARMD is the leading cause of vision loss and blindness among older individuals in the developed word. It is classified as either dry (nonneovascular) or wet (neovascular). ARMD7 is a wet form, in which new blood vessels form and break beneath the retina. This leakage causes permanent damage to surrounding retinal tissue, distorting and destroying central vision. Wet ARMD is more prevalent among Asians than Caucasians.
    Defects in HTRA1 are the cause of cerebral autosomal recessive arteriopathy with subcortical infarcts and leukoencephalopathy (CARASIL) [MIM:600142]. CARASIL is characterized by nonhypertensive cerebral small-vessel arteriopathy with subcortical infarcts, alopecia, and spondylosis, with an onset in early adulthood. On neuropathological examination, arteriosclerosis associated with intimal thickening and dense collagen fibers, loss of vascular smooth-muscle cells, and hyaline degeneration of the tunica media has been observed in cerebral small arteries.
  • Sequence similarities

    Belongs to the peptidase S1B family.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 Kazal-like domain.
    Contains 1 PDZ (DHR) domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • ARMD7 antibody
    • CARASIL antibody
    • High-temperature requirement A serine peptidase 1 antibody
    • HtrA antibody
    • HtrA serine peptidase 1 antibody
    • HTRA1 antibody
    • HTRA1_HUMAN antibody
    • IGFBP5 protease antibody
    • L56 antibody
    • ORF480 antibody
    • Protease serine 11 (IGF binding) antibody
    • protease serine 11 antibody
    • PRSS11 antibody
    • Serine protease 11 antibody
    • Serine protease HTRA1 antibody
    • Serine protease HTRA1 precursor antibody
    see all

Images

  • All lanes : Anti-htrA1 antibody [EPR14605(2)] (ab199529) at 1/5000 dilution

    Lane 1 : HepG2 (Human liver hepatocellular carcinoma) cell lysate
    Lane 2 : MCF7 (Human breast adenocarcinoma cell line) cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-htrA1 antibody [EPR14605(2)] (ab199529) at 1/1000 dilution + Human fetal liver tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 51 kDa
    Observed band size: 51 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling htrA1 with ab199529 at 1/1200 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 (green). Cytoplasm staining on MCF7cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

    The negative controls are as follows:-
    -ve control 1: - ab199529 at 1/1200 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: - ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (Human liver hepatocellular carcinoma) cells labeling htrA1 with ab199529 at 1/150 dilution (red), compared with a rabbit monoclonal IgG isotype control (ab172730, black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody (blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

  • htrA1 was immunoprecipitated from 1mg of HepG2 (Human liver hepatocellular carcinoma) whole cell extract with ab199529 at 1/40 dilution. Western blot was performed using ab199529 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: HepG2 whole cell extract 10 µg (Input).

    Lane 2: ab199529 IP in HepG2 whole cell extract.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199529 in HepG2 whole cell extract.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab199529 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human and mouse eyes)
Antigen retrieval step
Heat mediated
Permeabilization
No
Specification
Human and mouse eyes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Mar 23 2015

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