Key features and details
- Rabbit polyclonal to HtrA2 / Omi
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HtrA2 / Omi antibody
See all HtrA2 / Omi primary antibodies
DescriptionRabbit polyclonal to HtrA2 / Omi
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human HtrA2/ Omi.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
Purification notesIon exchange chromatography.
Our Abpromise guarantee covers the use of ab21307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 48 kDa.|
FunctionSerine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
Tissue specificityIsoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.
Involvement in diseaseDefects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 PDZ (DHR) domain.
DomainThe mature N-terminus is involved in the interaction with XIAP.
The PDZ domain mediates interaction with MXI2.
Cellular localizationMitochondrion intermembrane space. Mitochondrion membrane. Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.
- Information by UniProt
- High temperature requirement protein A2 antibody
- HTRA 2 antibody
- HtrA like serine protease antibody
Immunofluorescence of HtrA2 / Omi in Human colon tissue with ab21307 at 20 ug/mL.
Lane 1 : Anti-HtrA2 / Omi antibody (ab21307) at 0.5 µg/ml
Lane 2 : Anti-HtrA2 / Omi antibody (ab21307) at 1 µg/ml
All lanes : Human colon cell lysate
Predicted band size: 48 kDa
Western blot analysis of HtrA2 in human colon cell lysates with anti-HtrA2 at (A) 0.5 and (B) 1
ab21307 has been referenced in 1 publication.
- Li H et al. YAP Inhibits the Apoptosis and Migration of Human Rectal Cancer Cells via Suppression of JNK-Drp1-Mitochondrial Fission-HtrA2/Omi Pathways. Cell Physiol Biochem 44:2073-2089 (2017). PubMed: 29241219