Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
Purification notesIon exchange chromatography.
Our Abpromise guarantee covers the use of ab21307 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 48 kDa.|
FunctionSerine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
Tissue specificityIsoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.
Involvement in diseaseDefects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 PDZ (DHR) domain.
DomainThe mature N-terminus is involved in the interaction with XIAP.
The PDZ domain mediates interaction with MXI2.
Cellular localizationMitochondrion intermembrane space. Mitochondrion membrane. Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.
- Information by UniProt
- High temperature requirement protein A2 antibody
- HTRA 2 antibody
- HtrA like serine protease antibody
Immunofluorescence of HtrA2 / Omi in Human colon tissue with ab21307 at 20 ug/mL.
Lane 1 : Anti-HtrA2 / Omi antibody (ab21307) at 0.5 µg/ml
Lane 2 : Anti-HtrA2 / Omi antibody (ab21307) at 1 µg/ml
All lanes : Human colon cell lysate
Predicted band size: 48 kDa
Western blot analysis of HtrA2 in human colon cell lysates with anti-HtrA2 at (A) 0.5 and (B) 1