Key features and details
- Rabbit polyclonal to HtrA3 - Catalytic domain
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HtrA3 antibody - Catalytic domain
See all HtrA3 primary antibodies
DescriptionRabbit polyclonal to HtrA3 - Catalytic domain
Specificityab65911 recognizes both of the isoforms of HtrA3.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide based on the catalytic domain of human HtrA3.
- Extracts of SW 620 in Serum Free, or treated with TPA, IL 1-Alpha, or IL 1-Beta.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65911 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionProbable serine protease.
Tissue specificityWidely expressed, with highest levels in both adult and fetal heart, ovary, uterus placenta, and bladder. Isoform 1 is predominant in heart and skeletal muscle, whereas isoform 2 is predominant in placenta and kidney.
Sequence similaritiesBelongs to the peptidase S1B family.
Contains 1 IGFBP N-terminal domain.
Contains 1 Kazal-like domain.
Contains 1 PDZ (DHR) domain.
- Information by UniProt
- High temperature requirement factor A3 antibody
- High-temperature requirement factor A3 antibody
- HtrA serine peptidase 3 antibody
All lanes : Anti-HtrA3 antibody - Catalytic domain (ab65911) at 1/1000 dilution
Lane 1 : SW 620 with SF
Lane 2 : SW 620 with TPA
Lane 3 : SW 620 with IL 1-Alpha
Lane 4 : SW 620 with IL 1-Beta
Lane 5 : Molecular weight markers
Predicted band size: 50 , 38 kDa
Observed band size: 40,48 kDa why is the actual band size different from the predicted?
Multiple additonal bands are observed. We are unsure as to the identity of these extra bands.
ab65911 has been referenced in 1 publication.
- Russell TM & Johnson BJ Lyme disease spirochaetes possess an aggrecan-binding protease with aggrecanase activity. Mol Microbiol 90:228-40 (2013). PubMed: 23710801