Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-HuB+HuC+HuD antibody [16A11C1D4]
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DescriptionMouse monoclonal [16A11C1D4] to HuB+HuC+HuD
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Host speciesMouse
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Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
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Species reactivityReacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human HuB+HuC+HuD aa 233-244 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
Sequence:QAQRFRLDNLLN
Database link: Q12926 -
Positive control
- HuC Human recombinant protein fragment His-tagged; HuD Human recombinant protein; SH-SY5Y cell lysate; SH-SY5Y cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading... -
Purification notesThe purity of ab176106 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.
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ClonalityMonoclonal
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Clone number16A11C1D4
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IsotypeIgG2b
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Light chain typekappa
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab176106 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | Use a concentration of 1 µg/ml. (or use at assay dependent concentration). |
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| ICC/IF | Use a concentration of 1 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 40, 42 kDa. |
Images
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![Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)](https://www.abcam.com/ps/products/176/ab176106/Images/ab176106-203924-wb1.jpg)
Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)All lanes : Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106) at 1 µg/ml
Lane 1 : Human HuC protein fragment (ab91911) at 0.1 µg
Lane 2 : HuD human recombinant protein at 0.1 µg
Lane 3 : SH-SY5Y cell lysate at 20 µg
Secondary
All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 40, 42 kDaPredicted MW (kDa): HuB/ELAVL2: 39.5 kDa, HuC/ELAVL3: 39.5kDa, HuD/ELAVL4: 41.7kDa
Why the observed band size is different than the predicted band size: HuC protein (ab91911) is a fragment and has an N terminal His tag and is predicted to present at 24 kDa.
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![Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)](https://www.abcam.com/ps/products/176/ab176106/Images/ab176106-203927-icc1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)Immunocytochemistry with SH-SY5Y cells stained with ab176106 in red and DAPI in blue, as a counter stain.
Fixation: Paraformaldehyde fixed (4%, 20 minutes).
Permeabilization: Triton X-100 permeabilized (0.1%, 10 minutes).
Blocking: 10% Goat Serum/PBS (1 hour).
Primary: ab176106, 1 µg/ml (10% Goat Serum/PBS, 2 hours at room temperature or over night at 4°C).
Secondary: Alexa 594 goat anti-mouse H+L used at a 1/1000 dilution in 1%Goat Serum/PBS for 1hour at RT.
Washing: 3X 1% Goat Serum/PBS, 10 minutes per wash.
DAPI: 2 uM in 1% Goat Serum/PBS, 10 minutes.
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![ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)](https://www.abcam.com/ps/products/176/ab176106/Images/ab176106-203928-ELISA-1.jpg)
ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)Specificity of antibody using the ELISA platform.
Antibody concentration is at 1µg/mL the concentration of recombinant proteins is at 250ng/mL. ab176106 reacts specifically to HuC/ELAVL3 and HuD/ELAVL4 proteins. Reactivity with HuB protein is predicted by the immunogen sequence similarity.
References
ab176106 has not yet been referenced specifically in any publications.
