Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)

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Overview

  • Product name

    Anti-HuB+HuC+HuD antibody [16A11C1D4]

  • Description

    Mouse monoclonal [16A11C1D4] to HuB+HuC+HuD

  • Host species

    Mouse

  • Tested applications

    Suitable for: ELISA, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat

  • Immunogen

    Synthetic peptide corresponding to Human HuB+HuC+HuD aa 233-244 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue).
    Sequence:

    QAQRFRLDNLLN


    Database link: Q12926

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HuC Human recombinant protein fragment His-tagged; HuD Human recombinant protein; SH-SY5Y cell lysate; SH-SY5Y cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituent: 99% HEPES buffered saline
  • Concentration information loading...

  • Purification notes

    The purity of ab176106 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.

  • Clonality

    Monoclonal

  • Clone number

    16A11C1D4

  • Isotype

    IgG2b

  • Light chain type

    kappa

Applications

Our Abpromise guarantee covers the use of ab176106 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use a concentration of 1 µg/ml.

(or use at assay dependent concentration).
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 40, 42 kDa.

Images

  • Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
    Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
    All lanes : Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106) at 1 µg/ml

    Lane 1 : Human HuC protein fragment (ab91911) at 0.1 µg
    Lane 2 : HuD human recombinant protein at 0.1 µg
    Lane 3 : SH-SY5Y cell lysate at 20 µg

    Secondary
    All lanes : Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

    Predicted band size: 40, 42 kDa



    Predicted MW (kDa): HuB/ELAVL2: 39.5 kDa, HuC/ELAVL3: 39.5kDa, HuD/ELAVL4: 41.7kDa

    Why the observed band size is different than the predicted band size: HuC protein (ab91911) is a fragment and has an N terminal His tag and is predicted to present at 24 kDa.

  • Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
    Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)

    Immunocytochemistry with SH-SY5Y cells stained with ab176106 in red and DAPI in blue, as a counter stain.

    Fixation: Paraformaldehyde fixed (4%, 20 minutes).

    Permeabilization: Triton X-100 permeabilized (0.1%, 10 minutes).

    Blocking: 10% Goat Serum/PBS (1 hour).

    Primary: ab176106, 1 µg/ml (10% Goat Serum/PBS, 2 hours at room temperature or over night at 4°C).

    Secondary: Alexa 594 goat anti-mouse H+L used at a 1/1000 dilution in 1%Goat Serum/PBS for 1hour at RT.

    Washing: 3X 1% Goat Serum/PBS, 10 minutes per wash.

    DAPI: 2 uM in 1% Goat Serum/PBS, 10 minutes.

  • ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)
    ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106)

    Specificity of antibody using the ELISA platform.

    Antibody concentration is at 1µg/mL the concentration of recombinant proteins is at 250ng/mL. ab176106 reacts specifically to HuC/ELAVL3 and HuD/ELAVL4 proteins. Reactivity with HuB protein is predicted by the immunogen sequence similarity.

References

Publishing research using ab176106? Please let us know so that we can cite the reference in this datasheet.

ab176106 has not yet been referenced specifically in any publications.

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-HuB+HuC+HuD antibody [16A11C1D4]

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% triton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 10 2016

Immunohistochemistry (Frozen sections) abreview for Anti-HuB+HuC+HuD antibody [16A11C1D4]

 Excellent
Abreviews
This product is known to not work in this application or species.
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Mar 04 2016

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