Overview

  • Product name
    Anti-HUCE1 antibody [EPR10645(B)]
    See all HUCE1 primary antibodies
  • Description
    Rabbit monoclonal [EPR10645(B)] to HUCE1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human HUCE1 (Uniprot O43159) (the amino acid sequence is considered to be commercially sensitive).

  • Positive control
    • K562, HeLa, MCF7 and SH-SY5Y cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20ºC.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR10645(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155989 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 51 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function
      Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell: upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
    • Sequence similarities
      Belongs to the methyltransferase superfamily. RRP8 family.
    • Cellular localization
      Nucleus > nucleolus. Localizes at rDNA locus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cerebral protein 1 antibody
      • hypothetical protein LOC23378 antibody
      • KIAA0409 antibody
      • NML antibody
      • Nucleomethylin antibody
      • Ribosomal RNA processing 8 methyltransferase homolog antibody
      • Ribosomal RNA processing 8, methyltransferase, homolog (yeast) antibody
      • Ribosomal RNA processing protein 8 antibody
      • Ribosomal RNA-processing protein 8 antibody
      • RRP 8 antibody
      • RRP8 antibody
      • RRP8 methyltransferase homolog antibody
      • RRP8_HUMAN antibody
      see all

    Images

    • All lanes : Anti-HUCE1 antibody [EPR10645(B)] (ab155989) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : MCF7 cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 51 kDa

    References

    ab155989 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab155989.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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