Anti-HuD antibody [16C12AA10] (ab171448)
Key features and details
- Mouse monoclonal [16C12AA10] to HuD
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-HuD antibody [16C12AA10]
See all HuD primary antibodies -
Description
Mouse monoclonal [16C12AA10] to HuD -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human HuD aa 26-39 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:SNNRNCPSPMQTGA
Database link: P26378 -
Positive control
- ELAVL4 recombinant protein.
-
General notes
Product was previously marketed under the MitoSciences sub-brand.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
16C12AA10 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab171448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa. | |
ELISA | Use a concentration of 1 µg/ml. |
Target
-
Function
May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA. -
Tissue specificity
Brain. -
Sequence similarities
Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains. -
Post-translational
modificationsMethylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth. - Information by UniProt
-
Database links
- Entrez Gene: 1996 Human
- Omim: 168360 Human
- SwissProt: P26378 Human
- Unigene: 213050 Human
-
Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
- ELAV L4 antibody
- ELAV like 4 antibody
see all
Images
-
Anti-HuD antibody [16C12AA10] (ab171448) at 2 µg/ml + Human ELAV4 recombinant protein at 0.100000001490116 µg
Secondary
Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution
Predicted band size: 42 kDa -
Analysis demonstrating the specificity of ab171448 using the ELISA platform.
Antibody concentration is at 1 µg/mL, the concentration of recombinant proteins is at 250 ng/mL. The antibody reacts specifically to HuD/ELAV4 protein.
Datasheets and documents
References (0)
ab171448 has not yet been referenced specifically in any publications.