Name: Anti-HuD antibody [16C12AA10]
SKU: ab171448

Product name

Anti-HuD antibody [16C12AA10]
See all HuD primary antibodies
Description

Mouse monoclonal [16C12AA10] to HuD
Host species

Mouse
Tested applications

Suitable for: WB, ELISAmore details
Species reactivity

Reacts with: Human
Does not react with: Mouse, Rat, Cow
Immunogen

Synthetic peptide corresponding to Human HuD aa 26-39 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
Sequence:
SNNRNCPSPMQTGA

Database link: P26378

Run BLAST with Run BLAST with
Positive control
- ELAVL4 recombinant protein.
General notes

Product was previously marketed under the MitoSciences sub-brand.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.02% Sodium azide
Constituent: 99% HEPES buffered saline
Concentration information loading...
Clonality

Monoclonal
Clone number

16C12AA10
Isotype

IgG2b
Light chain type

kappa
Research areas

Our Abpromise guarantee covers the use of ab171448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa.
ELISA		Use a concentration of 1 µg/ml.

Function

May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.
Tissue specificity

Brain.
Sequence similarities

Belongs to the RRM elav family.
Contains 3 RRM (RNA recognition motif) domains.
Post-translational
modifications

Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth.
Target information above from: UniProt accession P26378 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1996 Human
- Omim: 168360 Human
- SwissProt: P26378 Human
- Unigene: 213050 Human
Alternative names
- ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
- ELAV L4 antibody
- ELAV like 4 antibody
- ELAV like protein 4 antibody
- ELAV-like protein 4 antibody
- ELAV4_HUMAN antibody
- Elavl4 antibody
- Embryonic lethal abnormal vision Drosophila homolog of like 4 antibody
- Hu antigen D antibody
- Hu-antigen D antibody
- HuD antibody
- Paraneoplastic encephalomyelitis antigen HuD antibody
- PNEM antibody
see all

Western blot - Anti-HuD antibody [16C12AA10] (ab171448)

Anti-HuD antibody [16C12AA10] (ab171448) at 2 µg/ml + Human ELAV4 recombinant protein at 0.100000001490116 µg

Secondary
Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

Predicted band size: 42 kDa
ELISA - Anti-HuD antibody [16C12AA10] (ab171448)

Analysis demonstrating the specificity of ab171448 using the ELISA platform.
Antibody concentration is at 1 µg/mL, the concentration of recombinant proteins is at 250 ng/mL. The antibody reacts specifically to HuD/ELAV4 protein.

ab171448 has not yet been referenced specifically in any publications.

Publishing research using ab171448? Please let us know so that we can cite the reference in this datasheet.

Anti-HuD antibody [16C12AA10] (ab171448)

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