Overview

  • Product name
    Anti-HuD antibody [16C12AA10]
    See all HuD primary antibodies
  • Description
    Mouse monoclonal [16C12AA10] to HuD
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human HuD aa 26-39 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    SNNRNCPSPMQTGA


    Database link: P26378

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • ELAVL4 recombinant protein.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab171448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa.
ELISA Use a concentration of 1 µg/ml.

Target

  • Function
    May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth.
  • Information by UniProt
  • Database links
    • Alternative names
      • ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
      • ELAV L4 antibody
      • ELAV like 4 antibody
      • ELAV like protein 4 antibody
      • ELAV-like protein 4 antibody
      • ELAV4_HUMAN antibody
      • Elavl4 antibody
      • Embryonic lethal abnormal vision Drosophila homolog of like 4 antibody
      • Hu antigen D antibody
      • Hu-antigen D antibody
      • HuD antibody
      • Paraneoplastic encephalomyelitis antigen HuD antibody
      • PNEM antibody
      see all

    Images

    • Western blot - Anti-HuD antibody [16C12AA10] (ab171448)
      Western blot - Anti-HuD antibody [16C12AA10] (ab171448)
      Anti-HuD antibody [16C12AA10] (ab171448) at 2 µg/ml + Human ELAV4 recombinant protein at 0.100000001490116 µg

      Secondary
      Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

      Predicted band size: 42 kDa

    • ELISA - Anti-HuD antibody [16C12AA10] (ab171448)
      ELISA - Anti-HuD antibody [16C12AA10] (ab171448)

      Analysis demonstrating the specificity of ab171448 using the ELISA platform.

      Antibody concentration is at 1 µg/mL, the concentration of recombinant proteins is at 250 ng/mL. The antibody reacts specifically to HuD/ELAV4 protein.

    References

    ab171448 has not yet been referenced specifically in any publications.

    Publishing research using ab171448? Please let us know so that we can cite the reference in this datasheet.

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