Anti-HuD antibody [16C12AA10] (ab171448)

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Western blot - Anti-HuD antibody [16C12AA10] (ab171448)
  • ELISA - Anti-HuD antibody [16C12AA10] (ab171448)

Key features and details

  • Mouse monoclonal [16C12AA10] to HuD
  • Suitable for: WB, ELISA
  • Reacts with: Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-HuD antibody [16C12AA10]
    See all HuD primary antibodies

  • Description

    Mouse monoclonal [16C12AA10] to HuD

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ELISAmore details

  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat, Cow

  • Immunogen

    Synthetic peptide corresponding to Human HuD aa 26-39 (N terminal) conjugated to keyhole limpet haemocyanin (Cysteine residue).
    Sequence:

    SNNRNCPSPMQTGA


    Database link: P26378
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • ELAVL4 recombinant protein.

  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab171448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Predicted molecular weight: 42 kDa.
ELISA Use a concentration of 1 µg/ml.

Target

  • Function

    May play a role in neuron-specific RNA processing. Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF and FOS mRNA.

  • Tissue specificity

    Brain.

  • Sequence similarities

    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.

  • Post-translational
    modifications

    Methylation at Arg-243 by CARM1 weakens protective binding to the 3'-UTR of CDKN1A mRNA and down-regulates CDKN1A protein expression, thereby maintaining cells in a proliferative state. Methylation is inhibited by NGF, which facilitates neurite outgrowth.
  • Information by UniProt

  • Database links

    • Alternative names

      • ELAV (embryonic lethal abnormal vision Drosophila) like 4 antibody
      • ELAV L4 antibody
      • ELAV like 4 antibody
      • ELAV like protein 4 antibody
      • ELAV-like protein 4 antibody
      • ELAV4_HUMAN antibody
      • Elavl4 antibody
      • Embryonic lethal abnormal vision Drosophila homolog of like 4 antibody
      • Hu antigen D antibody
      • Hu-antigen D antibody
      • HuD antibody
      • Paraneoplastic encephalomyelitis antigen HuD antibody
      • PNEM antibody
      see all

    Images

    • Western blot - Anti-HuD antibody [16C12AA10] (ab171448)
      Western blot - Anti-HuD antibody [16C12AA10] (ab171448)
      Anti-HuD antibody [16C12AA10] (ab171448) at 2 µg/ml + Human ELAV4 recombinant protein at 0.100000001490116 µg

      Secondary
      Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

      Predicted band size: 42 kDa

    • ELISA - Anti-HuD antibody [16C12AA10] (ab171448)
      ELISA - Anti-HuD antibody [16C12AA10] (ab171448)

      Analysis demonstrating the specificity of ab171448 using the ELISA platform.

      Antibody concentration is at 1 µg/mL, the concentration of recombinant proteins is at 250 ng/mL. The antibody reacts specifically to HuD/ELAV4 protein.

    References (0)

    Publishing research using ab171448? Please let us know so that we can cite the reference in this datasheet.

    ab171448 has not yet been referenced specifically in any publications.

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