Human 14,15 DHET ELISA Kit (ab175813)
- Datasheet
- References
- Protocols
Overview
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Product nameHuman 14,15 DHET ELISA Kit
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Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% overall 12 0.93% Inter-assay Sample n Mean SD CV% overall 12 1.31% -
Sample typeUrine
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Assay typeCompetitive
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Sensitivity3 pg/ml
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Range0.01 ng/ml - 1000 ng/ml
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Assay durationMultiple steps standard assay
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Species reactivityReacts with: Human
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Product overview
Abcam's 14,15 DHET competitive in vitro ELISA Kit for Human Urine is designed for determination of free and glucuronidated 14,15-DHET levels in urine.
This competitive ELISA kit is based on competition between the 14,15-DHET epitope and the 14,15-DHET-HRP conjugate for a limited number of binding sites available from the anti-14,15-DHET antibody; which is coated to the wells of the 96 well ELISA plate. The conjugate concentration is held as a constant in each well, while the concentration of the 14,15-DHET is variable, based on the concentration of the sample or standard. Thus the amount of the 14,15-DHET conjugate which is able to bind to each of the wells is inversely proportional to the concentration of 14,15-DHET in the standard or sample. The amount of the conjugate which is bound to each well is then determined by the amount of color obtained, when TMB is added. The TMB reacts with the HRP available in the well. With the addition of sulfuric acid, the blue colored product is converted into a yellow colored product, which can be read on a plate reader at 450 nm.
This competitive ELISA kit is for determination of free and glucuronidated 14,15-DHET levels in urine. The 14,15-DHET is a representative metabolite of soluble epoxide hydrolase-mediated metabolism of EETs, which are generated by arachidonic acid epoxygenase activity of cytochromes P450. 14,15-DHET level exhibited strong positive correlation with hypertension in rat and human and brain injury and stroke in rodents. Human urine and blood 14,15-DHET levels were measured using the 14,15-DHET ELISA kit.
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Tested applicationsSuitable for: Competitive ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components 1 x 96 tests 14,15-DHET Standard 1 x 2µl 14,15-DHET-HRP Conjugates 1 x 12µl Beta-Glucuronidase enzyme 1 x 8mg HRP Buffer 1 x 15ml Pre-coated 96-Well Microplate 1 unit Sample Dilution Buffer 1 x 25ml TMB Substrate 1 x 24ml Wash Buffer Solution 1 x 25ml -
Research areas
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Alternative names
- 14,15-dihydroxy-5Z,8Z,11Z-eicosatrienoic acid
Applications
Our Abpromise guarantee covers the use of ab175813 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Competitive ELISA | Use at an assay dependent concentration. |
Images
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Typical Standard CurveRepresentative standard curve using ab175813 14,15 DHET ELISA Kit for Human Urine.
Protocols
References
ab175813 has not yet been referenced specifically in any publications.
