Overview

  • Product name

    Human AACT Matched Antibody Pair Kit
    See all AACT kits
  • Detection method

    Colorimetric
  • Assay type

    ELISA set
  • Range

    500 pg/ml - 32000 pg/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human AACT.


    Matched antibody pair kits and reagents deliver consistent, specific, and sensitive results.



    • Batch-to-batch consistency: only recombinant monoclonal antibodies are used in our matched antibody pairs.

    • Specificity: antibody pairs are screened in plasma and serum to ensure specificity in complex samples.

    • Sensitivity: benchmarked against commercially available antibody pairs to ensure equivalent or superior performance compared with the competition.


    Additional buffers and plates are required for the assay. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).


    For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA® (ab217779) which uses the same antibody pair.


    To receive an electronic copy of the Certificate of Analysis, please send an email with "CoA for matched antibody pair kit" in the subject line and the desired product number and lot number in the body of the email.

  • Tested applications

    Suitable for: ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 10 x 96 tests 2 x 96 tests
    Human AACT Capture Antibody 1 x 100µg 1 x 20µg
    Human AACT Detector Antibody 1 x 25µg 1 x 5µg
    Human AACT Lyophilized Protein 1 vial 1 vial
  • Research areas

  • Function

    Although its physiological function is unclear, it can inhibit neutrophil cathepsin G and mast cell chymase, both of which can convert angiotensin-1 to the active angiotensin-2.
  • Tissue specificity

    Plasma. Synthesized in the liver. Like the related alpha-1-antitrypsin, its concentration increases in the acute phase of inflammation or infection. Found in the amyloid plaques from the hippocampus of Alzheimer disease brains.
  • Involvement in disease

    Defects in SERPINA3 may be a cause of chronic obstructive pulmonary disease (COPD) [MIM:107280].
  • Sequence similarities

    Belongs to the serpin family.
  • Domain

    The reactive center loop (RCL) extends out from the body of the protein and directs binding to the target protease. The protease cleaves the serpin at the reactive site within the RCL, establishing a covalent linkage between the carboxyl group of the serpin reactive site and the serine hydroxyl of the protease. The resulting inactive serpin-protease complex is highly stable.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Alternative names

    • SERPINA3
    • AACT
    • AACT_HUMAN
    • ACT
    • alpha 1 Antichymotrypsin
    • Alpha-1-antichymotrypsin His-Pro-less
    • Antichymotrypsin
    • Cell growth inhibiting gene 24/25 protein
    • Cell growth-inhibiting gene 24/25 protein
    • GIG24
    • GIG25
    • Growth inhibiting protein 24
    • Growth inhibiting protein 25
    • MGC88254
    • Serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase, antitrypsin) member 3
    • Serine (or cysteine) proteinase inhibitor clade A member 3
    • Serine proteinase inhibitor clade A member 3
    • Serpin A3
    • Serpin family A member 3
    • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 3
    • SERPINA3
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab221445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Images

  • Standard calibration curve. Background substracted values graphed.

Protocols

References

ab221445 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab221445.
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