• Product name
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma, Other biological fluids, Tissue Extracts
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 5 pg/ml
  • Range
    0.78 ng/ml - 50 ng/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Human ACE (CD143) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human ACE (CD143) in cell culture supernatants, serum, plasma (heparin) and saliva.

    A ACE (CD143) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for ACE (CD143) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human ACE (CD143) amount of sample captured in plate.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    ABC Diluent Buffer 1 x 12ml
    Antibody Diluent Buffer 1 x 12ml
    Anti-Human ACE Antibody Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti-human ACE antibody 1 x 130µl
    Lyophilized Recombinant Human ACE standard 2 x 50ng
    Plate Seal 1 x 4 units
    Sample Diluent Buffer 1 x 30ml
    TMB Color Developing Agent 1 x 10ml
    TMB Stop Solution 1 x 10ml
  • Research areas
  • Function
    Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
  • Tissue specificity
    Ubiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
  • Involvement in disease
    Ischemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
    Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
    Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
  • Sequence similarities
    Belongs to the peptidase M2 family.
  • Post-translational
    Phosphorylated by CK2 on Ser-1299; which allows membrane retention.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • ACE
    • ACE 1
    • ACE T
    • ACE1
    • Angiotensin converting enzyme somatic isoform
    • Angiotensin converting enzyme testis specific isoform
    • Angiotensin I converting enzyme
    • Angiotensin I converting enzyme 1
    • Angiotensin I converting enzyme peptidyl dipeptidase A 1
    • angiotensin I converting enzyme peptidyl-dipeptidase A 1 transcript
    • Angiotensin-converting enzyme
    • Carboxycathepsin
    • CD 143
    • CD143
    • CD143 antigen
    • DCP
    • DCP 1
    • DCP1
    • Dipeptidyl carboxypeptidase 1
    • Dipeptidyl carboxypeptidase I
    • Kininase II
    • MGC26566
    • MVCD3
    • Peptidase P
    • Peptidyl dipeptidase A
    • soluble form
    • Testicular ECA
    see all
  • Database links


Our Abpromise guarantee covers the use of ab119577 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • ACE measured in various fluids showing quantity (pg) per mL of tested sample



This product has been referenced in:
  • Sharma JN  et al. Altered activities of kininase II, an angiotensin converting enzyme, prekallikrein, and nitric oxide in Kuwaiti patients with type 2 diabetes. Int J Immunopathol Pharmacol 28:240-6 (2015). Sandwich ELISA ; Human . Read more (PubMed: 25964383) »

See 1 Publication for this product

Customer reviews and Q&As

For ab119577 Human ACE ELISA Kit, this is tested and covered by the Abpromise guarantee for tissue samples. However, I am sorry we have no testing data specifically for muscle samples. Therefore, the sample preparation will need to be individually opti...

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I can now confirm that the antibodies used in the kit are Ace specific, and these should not be cross reacting with ACE2 protein.
I have also done a quick sequence compatibility search between Ace and Ace2 protein, the sequences don’t show high ho...

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To detect ACE concentration in urine samples

Good Excellent 5/5 (Ease of Use)
ab119577 - ACE (CD143) Human ELISA Kit allows detection of ACE in urine samples. The urine samples could be used directly without dilution for the experiment. The sample could be prepared in the similar way as mentioned for serum/plasma in the protocol booklet.

Abcam user community

Verified customer

Submitted Apr 12 2016

I am sorry to confirm that sodium citrate will affect the results generated with this kit. Sodium citrate has chelating properties and will bind to ACE.

I cannot recommend to use samples from sodium citrate tubes.

Vielen Dank für Ihre Antwort.

Es tut mir leid, dass wir die Ursache des Problems nicht herausfinden konnten.Wir werdenauch die getesten Proben auf dem Datenblatt updaten und klarer beschreiben.

Ich habe unsere Finanzabteilung ...

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Vielen Dank für Ihre Antworten.Leider kann ich nicht bestimmen, woher das Problem mit diesem Kit kommen könnte. Ich werde Ihnen deshalb gerne dieses Kit ersetzen - entweder mit einem neuen kostenlosen Kit oder mit einer Gutschrift/Rücker...

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Vielen Dank für Ihre Anfrage und Ihre Geduld, während ich mit dem Labor in Kontakt war.

Es tut mir leid, dass Sie Probleme mit diesem Kit von uns hatten. In der Tat sind die Daten ziemlich variabel. Sogar die Duplikate sind sehr we...

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Thank you for contacting us.

I have been in touch with the development group regarding finding a conversion factor for converting to internation units of ACE. As this product measures total ACE amounts against a known recombinant standard ...

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Thank you for contacting us.

EDTA is not recommended as an anticoagulant because of its chelating properties. It may capture Zn ions from the ACE protein and cause changes in the structure that could inhibit antibody binding and lead to reduc...

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Gracias por contactarnos.

Mi compañera de Servicio al Cliente me ha remitido tu correo.

Nuestros productos se envían en bloques de hielo como medida simplemente preventiva, para mantenerlos a una temperatura constante ...

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