Key features and details
- Sensitivity: 5 pg/ml
- Range: 0.78 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Hep Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman ACE ELISA Kit (CD143)
See all Angiotensin Converting Enzyme 1 kits
Intra-assay Sample n Mean SD CV% 1 16 1.278ng/ml 0.626 = 4.9% 2 16 6.839ng/ml 0.465 = 6.8% 3 16 24.157ng/ml 1.087 = 4.5% Inter-assay Sample n Mean SD CV% 1 24 1.358ng/ml 0.086 = 6.5% 2 24 6.35ng/ml 0.482 = 7.6% 3 24 25.528ng/ml 1.225 = 4.8%
Sample typeCell culture supernatant, Saliva, Serum, Hep Plasma
Assay typeSandwich (quantitative)
Sensitivity< 5 pg/ml
Range0.78 ng/ml - 50 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Human ACE (CD143) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human ACE (CD143) in cell culture supernatants, serum, plasma (heparin) and saliva.
An ACE (CD143) specific goat polyclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat, specific for ACE (CD143) is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human ACE (CD143) amount of sample captured in plate.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human ACE Antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-human ACE antibody 1 x 100µl Lyophilized Recombinant Human ACE standard 2 x 50ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml
FunctionConverts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
Tissue specificityUbiquitously expressed, with highest levels in lung, kidney, heart, gastrointestinal system and prostate. Isoform Testis-specific is expressed in spermatocytes and adult testis.
Involvement in diseaseIschemic stroke (ISCHSTR) [MIM:601367]: A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Renal tubular dysgenesis (RTD) [MIM:267430]: Autosomal recessive severe disorder of renal tubular development characterized by persistent fetal anuria and perinatal death, probably due to pulmonary hypoplasia from early-onset oligohydramnios (the Potter phenotype). Note=The disease is caused by mutations affecting the gene represented in this entry.
Microvascular complications of diabetes 3 (MVCD3) [MIM:612624]: Pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Intracerebral hemorrhage (ICH) [MIM:614519]: A pathological condition characterized by bleeding into one or both cerebral hemispheres including the basal ganglia and the cerebral cortex. It is often associated with hypertension and craniocerebral trauma. Intracerebral bleeding is a common cause of stroke. Note=Disease susceptibility is associated with variations affecting the gene represented in this entry.
Sequence similaritiesBelongs to the peptidase M2 family.
modificationsPhosphorylated by CK2 on Ser-1299; which allows membrane retention.
Cellular localizationSecreted and Cell membrane.
- Information by UniProt
- ACE 1
- ACE T
ab119577 has been referenced in 2 publications.
- Desai T et al. Effects of short-term continuous Montmorency tart cherry juice supplementation in participants with metabolic syndrome. Eur J Nutr 60:1587-1603 (2021). PubMed: 32789528
- Sharma JN et al. Altered activities of kininase II, an angiotensin converting enzyme, prekallikrein, and nitric oxide in Kuwaiti patients with type 2 diabetes. Int J Immunopathol Pharmacol 28:240-6 (2015). Sandwich ELISA ; Human . PubMed: 25964383