Overview

  • Product name

    Human ADAM12 ELISA Kit (MCMP)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 10%
  • Sample type

    Cell culture supernatant, Urine, Serum, Hep Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 10 pg/ml
  • Range

    156 pg/ml - 10000 pg/ml
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Human ADAM12 (MCMP) in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human ADAM12 in cell culture supernatants, serum, plasma (heparin) and urine.

    An ADAM12 (MCMP) specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards (CHO, R29-S513) and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for ADAM12 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human ADAM12 amount of sample captured in plate. This kit recognizes pro-ADAM12, mature ADAM12, and ADAM12/TIMP-3 complex.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab171346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Representative standard curve using ab171346

Protocols

References

ab171346 has not yet been referenced specifically in any publications.

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