Overview

  • Product name

    Human ADAM9 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    45 pg/ml
  • Range

    45 pg/ml - 12000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96.06 88% - 104%
    Plasma 91.5 81% - 101%
    Cell culture media 122.2 114% - 130%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s ADAM9 Human ELISA Kit (ab193690) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human ADAM9 in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human ADAM9 coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures ADAM9 present in the samples. The wells are washed and biotinylated anti-Human ADAM9 antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of ADAM9 bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    400X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Detection Antibody ADAM9 (biotinylated anti-Human ADAM9) 2 vials
    Human ADAM9 Standards (lyophilized) 2 vials
    Pre-coated Human ADAM9 Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function

    Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP.
  • Tissue specificity

    Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart.
  • Involvement in disease

    Cone-rod dystrophy 9
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names

    • ADAM 9
    • ADAM metallopeptidase domain 9
    • Adam9
    • ADAM9_HUMAN
    • Cellular disintegrin-related protein
    • Cone rod dystrophy 9
    • CORD9
    • Disintegrin and metalloproteinase domain-containing protein 9
    • MCMP
    • MDC9
    • Meltrin-gamma
    • Metalloprotease/disintegrin/cysteine-rich protein 9
    • Mltng
    • Myeloma cell metalloproteinase
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard Curve in Assay Diluent A using ab193690 ADAM9 Human ELISA Kit.

    Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

  • Standard Curve in Assay Diluent B using ab193690 ADAM9 Human ELISA Kit.

    Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193690 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab193690.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up