Human ADAMTS1 ELISA Kit (ab213751)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 93.8 pg/ml - 6000 pg/ml
- Sample type: Cell culture supernatant, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human ADAMTS1 ELISA Kit
See all ADAMTS1 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 145pg/ml 8.84 = 6.1% 2 16 681pg/ml 37.45 = 5.5% 3 16 2886pg/ml 207.79 = 7.2% Inter-assay Sample n Mean SD CV% 1 24 132pg/ml 10.56 = 8% 2 24 732pg/ml 51.97 = 7.1% 3 24 2843pg/ml 213.22 = 7.5% -
Sample type
Cell culture supernatant, Serum, Hep Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
93.8 pg/ml - 6000 pg/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human ADAMTS1 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213751) is designed for the quantitative measurement of Human ADAMTS1 in cell culture supernatants, serum and plasma (heparin).
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS1 has been pre-coated onto 96-well plates. Standards (Expression system for standard: NSO; Immunogen sequence: F253-A734) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS1 amount of sample captured in plate.
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Notes
ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. The ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin.
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml 1 x 12ml Adhesive Plate Seal 4 units 4 units Antibody Diluent Buffer Green Cap 1 x 12ml 1 x 12ml Anti-Human ADAMTS1 coated Microplate (12 x 8 wells) 1 unit 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl 1 x 100µl Biotinylated anti- Human ADAMTS1 antibody 1 x 100µl 1 x 100µl Lyophilized recombinant Human ADAMTS1 standard 2 vials 2 vials Sample Diluent Buffer Green Cap 1 x 30ml 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml 1 x 10ml Wash Buffer (25X) 1 x 20ml 1 x 20ml -
Research areas
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 1
- ADAM-TS 1
- ADAM-TS1
see all -
Database links
- Entrez Gene: 9510 Human
- Entrez Gene: 9510 Human
- Omim: 605174 Human
- SwissProt: Q9UHI8 Human
- SwissProt: Q9UHI8 Human
- Unigene: 643357 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab213751 has not yet been referenced specifically in any publications.