Overview

  • Product name

    Human ADAMTS4 ELISA Kit
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    1 16 5.9ng/ml 0.248 4.2%
    2 16 12ng/ml 0.612 5.1%
    3 16 21ng/ml 1.2 5.7%
    Inter-assay
    Sample n Mean SD CV%
    1 24 6.5ng/ml 0.377 5.8%
    2 24 14ng/ml 0.882 6.3%
    3 24 23ng/ml 1.66 7.2%
  • Sample type

    Cell culture supernatant, Serum, Cell Lysate, Hep Plasma, EDTA Plasma, Tissue Homogenate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 20 pg/ml
  • Range

    625 pg/ml - 40000 pg/ml
  • Assay time

    3h 30m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Human ADAMTS4 Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213753) is designed for the quantitative measurement of Human ADAMTS4 in cell culture supernatants, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).


    The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS4 has been pre-coated onto 96-well plates. Standards (Expression system for standard: CHO; Immunogen sequence: F213-C685) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ADAMTS4 amount of sample captured in plate.

  • Notes

    ADAMTS4, a disintegrin and metalloproteinase with thrombospondin motifs 4 is an enzyme that in humans is encoded by the ADAMTS4 gene. ADAMTS4 is a member of the large ADAMTS family of zinc-dependent proteases. The human ADAMTS4 gene is mapped to chromosome 1 by somatic cell hybrid analysis. The enzyme encoded by this gene lacks a C-terminal TS motif. It is responsible for the degradation of aggrecan, a major proteoglycan of cartilage, and brevican, a brain-specific extracellular matrix protein. The cleavage of aggrecan and brevican suggests key roles of this enzyme in arthritic disease and in the central nervous system, potentially, in the progression of glioma.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests
    ABC Diluent Buffer Blue Cap 1 x 12ml
    Adhesive Plate Seal 4 units
    Antibody Diluent Buffer Green Cap 1 x 12ml
    Anti-Human ADAMTS4 coated Microplate (12 x 8 wells) 1 unit
    Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl
    Biotinylated anti- Human ADAMTS4 antibody 1 x 130µl
    Lyophilized recombinant Human ADAMTS4 standard 2 vials
    Sample Diluent Buffer Green Cap 1 x 30ml
    TMB Color Developing Agent Black Cap 1 x 10ml
    TMB Stop Solution Yellow Cap 1 x 10ml
  • Research areas

  • Function

    Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
    -Ala-393' site.
  • Tissue specificity

    Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 1 TSP type-1 domain.
  • Domain

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Alternative names

    • A disintegrin and metalloproteinase with thrombospondin motifs 4
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 4
    • ADAM metallopeptidase with thrombospondin type 1 motif 4
    • ADAM TS 4
    • ADAM TS4
    • ADAM-TS 4
    • ADAM-TS4
    • ADAMTS 2
    • ADAMTS 4
    • ADAMTS-4
    • ADAMTS2
    • ADAMTS4
    • ADAMTS4 protein
    • ADMP 1
    • ADMP-1
    • ADMP1
    • Aggrecanase 1
    • Aggrecanase-1
    • Aggrecanase1
    • ATS4_HUMAN
    • KIAA0688
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab213753 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Human ADAMTS4 ELISA Kit (ab213753) Standard Curve.

Protocols

References

ab213753 has not yet been referenced specifically in any publications.

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