Key features and details
- Sample type: Cit plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Indirect
- Reacts with: Human
Product nameHuman Anti-Adenovirus IgM ELISA Kit
Intra-assay Sample n Mean SD CV% Serum 20 11.3% Inter-assay Sample n Mean SD CV% Serum 12 8.3%
Sample typeSerum, Hep Plasma, Cit plasma
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s anti-Adenovirus IgM Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate qualitative measurement of IgM class antibodies against Adenovirus in Human serum and plasma.
A 96-well plate has been precoated with Adenovirus antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgM conjugate is added to the wells, which binds to the immobilized Adenovirus-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of Adenovirus IgM sample captured in plate.
Storage instructionsStore at +4°C. Please refer to protocols.
Components Identifier 1 x 96 tests 1 Strip holder 1 unit 20X Washing Solution White cap 1 x 50ml Adenovirus (IgM) Coated Microplate (12 x 8 wells) 1 unit Adenovirus anti-IgM HRP Conjugate Black cap 1 x 20ml Adenovirus IgM Cut-off Control Green cap 1 x 3ml Adenovirus IgM Negative Control Blue cap 1 x 2ml Adenovirus IgM Positive Control Red cap 1 x 2ml Cover foil 1 unit IgM Sample Diluent White cap 1 x 100ml Stop Solution red cap 1 x 15ml TMB Substrate Solution Yellow cap 1 x 15ml
ab108706 has been referenced in 1 publication.
- Guo L et al. Identification of a nonstructural DNA-binding protein (DBP) as an antigen with diagnostic potential for human adenovirus. PLoS One 8:e56708 (2013). PubMed: 23516396