Overview

  • Product name

    Human Albumin ELISA Kit
    See all Albumin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 3.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 8.6%
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Competitive
  • Sensitivity

    = 1.5 µg/ml
  • Range

    1.563 µg/ml - 100 µg/ml
  • Recovery

    97 %

  • Assay time

    2h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Albumin Human in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the measurement of albumin in Human plasma and serum.


    An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated albumin is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of albumin captured in plate.


    Get results in 90 minutes with Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108787.

Protocols

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

We have used human plasma at a dilution of 10,000x. Rat plasma was also tested at 50x but there was no cross-reactivity.

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Answer

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining consistent results from this kit.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee forELISA and human samples. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I have enclosed a technical questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily.

I would appreciate if you could are also able toprovidestandard curve data from each of the kits you have used, which would help us to assess the results.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



A. General Information
- Catalogue number:

- Lot number:

- Purchase order number/ order date or preferably the Abcam order number:

- How was the kit stored?


B. Description of the problem
- Are the signals you obtained with control or standard satisfactory? (Yes/No);


- Are the sample readings as expected or inconsistent?


- Are the blank readings as expected?

- Other (e.g. missing components)


C. What do you think the problem is caused by? E.g. enzyme is faulty, developer is faulty or other component that you think might be faulty.



D. Sample
- Sample Type (e.g. Cell culture supernatant, Cell lysates, Tissue lysates or Serum samples)


- Sample Species (e.g. human, mouse or rat)


- Storage (at what temperature and for how long were the samples stored?)

- Sample treatment, if any



- Sometime proteins in samples interfere with readings. Have you removed proteins?


- Sample preparation


E. Protocol used:
Did you follow the protocol exactly as given on the datasheet?


F. Optimization attempts (problem solving); please describe any deviations from the protocol.



G. Have you used the same kit successfully before?


H. Do you obtain the same results every time?


I. Additional Notes; (any additional information you would like to provide)



J. Results:

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Answer

Thank you very much for your call last week and for your patience while I have worked on your enquiry.

The lab has senta vial of the wash buffer, which we are sending to you free of charge on the order ***. This vial should arrive tomorrow.

I apologize for the delay in getting this for you, but please let me know if you have any questions or need anything else and I'll be happy to help.

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Answer

Thank you very much for your call this week and for your patience while I've been in touch with the lab.

The lab scientists do not recommend using a different buffer with this kit, as the results will be less than optimal. I'm arranging for a free of charge bottle of the wash buffer to be sent to you. Could you let me know your original order or PO number?

Please let me know if there is anything else that we can do for you, and I'll be happy to help.

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Question
Answer

DISCOUNT CODE: XXXXXXX
Expiration date: 15 June 2012

I am very pleased to hear you would like to accept our offer and test ab108787 with cell culture supernatant. This code will give you 1 free Kit before the expiration date. To redeem this offer, please submit an Abreview for Supernatant and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your email. Dr Tanya is out of office this week so I am dealing with her inquiries.
I am happy in issuing a discount code. Could you send the email and contact address of end user?
Cheers!

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Answer

Hope you are well?
Thank you for your enquiry and interest.
We would be very much interested in these data. Will the customer measure the secreted Albumin into the medium? Would it be possible to get some further information about the experimental set up?
Typically, researchers can submit a product review via an online submission form. At this time we do not have an Abreview form available for submitting reviews on this product. As an alternative, please email your review of ab108787 to me at First.Last@abcam.com.
In the review, please include:
1) A brief description of your experimental procedures
2) An attached JPEG or PNG formatted image illustrating
your data
2) Your views on the results
3) If you would like your name to be shown with your
review or if you prefer to be anonymous
Regarding your image, please note any descriptive details that will help other researchers interpret your results.
You will be awarded the same number of points as if you had submitted the review online using a standard Abreview form. You will receive 120 points for text with an additional 100 points for including an image. These points can be used for discounts on future purchases or Amazon gift cards.
For further information regarding Abreviews, please see https://www.abcam.com/index.html?pageconfig=resource&rid=10332. Thanks in advance for submitting your review. Feel free to contact me if you have any further questions.
Please reply to this email if you have any questions. If your customer would like to go ahead, then could you please pass his/her details to me so that I can issue the code as soon as possible.

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Answer

Thank you for your inquiry and you positive feedback.

Please see my answers to your questions below:

1. I can confirm that the kits ab108902 (Transferrin Human ELISA Kit) and ab108788 (Albumin Human ELISA kit) were especially optimised for cell culture samples and I recommend to choose them for the experiment.

2. We do offer bulk oder discounts. If you order 5 or more products you will get a discount. For 6 products you will currently get a 5% discount. Please do not forget to mention that when ordering. If you are planning to order more than 10 products, please contact our customer service department with the numbers and they might be able to offer you a higher discount.

3. I can confirm that c stands for competitive ELISA and s stands for sandwich ELISA. In a competitive ELISA the competitor is labeled with biotin and the amount of the bound competitor is measured. This means that a high OD indicates a low amount of the protein of interest in your sample. A low OD means that the competitor has been ousted and there is a high amount of the protein of interest in the sample.

4. I can confirm that the main difference between these two kits is the sensitivity. Both are suitable for cell culture supernatant, urine, serum, plasma.

Our Abpromise guarantee applies to all these kits.

https://www.abcam.com/abpromise

I hope this information is helpful and wish you good luck with your experiments. Please do not hesitate to contact me again with any further questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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