Overview

  • Product name
    Human Albumin ELISA Kit
    See all Albumin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.4%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    = 1.9 ng/ml
  • Range
    3.125 ng/ml - 200 ng/ml
  • Recovery

    97 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Human Albumin in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of albumin levels in urine, saliva, milk, cerebrospinal fluids and cell culture samples.


    An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.


    Get results in 90 minutes with Human Serum Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.


    Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.


     

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ab108788 Albumin Human ELISA kit
    ab108788 Albumin Human ELISA kit

    Concentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).

  • ab108788 Albumin Human ELISA kit
    ab108788 Albumin Human ELISA kit

    Albumin in HepG2 (diluted 1:50-200) and HeLa (undiluted) supernatants, heparin-treated plasma and serum (5e5-5e6), urine (100-500), milk (5000-30000), saliva (200-2000) was measured in duplicates (+/- SD).

  • ab108788 Albumin Human ELISA kit
    ab108788 Albumin Human ELISA kit

    Standard curve with background signal subtracted (duplicates; +/- SD).

  • ab108788 Human Albumin
    ab108788 Human Albumin

    Human albumin measured in various samples showing mass (microgram) per mL sample tested

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108788

Protocols

References

This product has been referenced in:
  • Le BD  et al. Three-Dimensional Hepatocellular Carcinoma/Fibroblast Model on a Nanofibrous Membrane Mimics Tumor Cell Phenotypic Changes and Anticancer Drug Resistance. Nanomaterials (Basel) 8:N/A (2018). Read more (PubMed: 29370123) »
  • Takacs CN  et al. Differential Regulation of Lipoprotein and Hepatitis C Virus Secretion by Rab1b. Cell Rep 21:431-441 (2017). Read more (PubMed: 29020629) »

See all 4 Publications for this product

Publishing research using ab108788? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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8 reviews or Q&A

10
Votes: Highest First

We are looking to run a total protein assay on milk samples that have been diluted to be ran on this kit as well. Can you tell me if the Diluent N has any components that would interfere with a standard protein assay?

Thank you for your inquiry. Ab108788 is validated for use with milk samples. The Diluent N does not contain BSA or casein but a BSA like protein. It does not interfere with human albumin measurement or cross react.

I would like to know if this kit is also good for plasma.

The albumin ELISA ab108788 is designed to be able to detect the relatively low amounts (compared to serum and plasma) of albumin in the sample types listed on the datasheet: urine, saliva, milk, cerebrospinal fluid and cell culture supernatants. It wil...

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Does the "10X Diluent Concentrate N" contain BSA or casein?

The laboratory has confirmed that the "10X Diluent Concentrate N" does not contain BSA or casein. It contains other proteins that have no cross-reactivity with human albumin.

Is it possible to purchase Nx1 thinner for use with ab10878?

Yes, we added this diluent to our catalog: Diluent Concentrate (10x) ab172376.

Although this kit has not been tested in cell or tissue lysates I want to test it with cells removed from the matrix. Are there any lysis buffers that I should avoid that may interfere with the kit?

While this kit as not been tested with tissue or cell lysates, in general, a lysis buffer containg a low concentration of triton-X-100 or SP-40 (less than 1 %) will not affect the assay.

Does ab108788 cross-react with horse serum albumin or BSA?


The source of the kit ab108788 informed me that they have never tested this kit with horse serum. They have tested it with BSA and they did not see any significant cross-reactivity.

I run an assay yesterday using the Albumin Human ELISA kit (ab108788).

Unfortunately, it was saturated. Is it possible to order the antibodies separately, that way I can re-run the experiment?

The antibodies used in this kit are optimized for use in kit only. These are not available separately. We have other antibodies available in our catalogue that can be used to develop the assay e.g. ab10241/ ab2406, ab117455

Abreview for Albumin Human ELISA kit

 Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2 cells)
Specification: HepG2 cells
Amount of Sample: 0.2ug albumin standard µg
Type of Kit Used: ELISA
Positive control: Provided Albumin Standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human albumin. Did not recognize bovine albumin in FBS.
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Submitted Jul 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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