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    human-albumin-elisa-kit-ab108788.pdf

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Cardiovascular Blood Serum Proteins
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Human Albumin ELISA Kit (ab108788)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (1)Q&A (7)References (36)

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ab108788 Albumin Human ELISA kit
  • ab108788 Albumin Human ELISA kit
  • Typical Standard Curve for ab108788 Human Albumin ELISA kit

Key features and details

  • Sensitivity: 0.94 ng/ml
  • Range: 12.5 ng/ml - 100 ng/ml
  • Sample type: Cell culture supernatant, Cerebral Spinal Fluid, Milk, Saliva, Urine
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

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Overview

  • Product name

    Human Albumin ELISA Kit
    See all Albumin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5%
    Inter-assay
    Sample n Mean SD CV%
    Overall 9.6%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.94 ng/ml
  • Range

    12.5 ng/ml - 100 ng/ml
  • Recovery

    97 %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Albumin ELISA kit (ab108788) is a sandwich ELISA kit designed for the quantitative measurement of albumin levels in urine, saliva, milk, cerebrospinal fluids and cell culture samples.


    An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.


    Get results in 90 minutes with Human Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.


    Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.


     


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent N Concentrate 1 x 30ml
    20X Wash Buffer Concentrate 2 x 30ml
    40X Biotinylated Human Albumin Antibody 1 x 150µl
    Albumin Microplate (12 x 8 well strips) 1 unit
    Albumin Standard 1 vial
    Chromogen Substrate 1 x 7ml
    Sealing Tapes 3 units
    Stop Solution 1 x 11ml
  • Research areas

    • Cardiovascular
    • Blood
    • Serum Proteins
    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Other Chaperones
    • Stem Cells
    • Lineage Markers
    • Endoderm
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Developmental Biology
    • Lineage specification
    • Endoderm
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Cardiovascular ELISA kits
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Signal transduction proteins ELISA kits
    • Metabolism
    • Types of disease
    • Metabolic disorders
    • Neuroscience
    • Processes
  • Function

    Serum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
  • Tissue specificity

    Plasma.
  • Involvement in disease

    Defects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
  • Sequence similarities

    Belongs to the ALB/AFP/VDB family.
    Contains 3 albumin domains.
  • Post-translational
    modifications

    Kenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
    Glycated in diabetic patients.
    Phosphorylation sites are present in the extracelllular medium.
    Acetylated on Lys-223 by acetylsalicylic acid.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession P02768 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • alb
    • ALBU_HUMAN
    • Albumin (32 AA)
    • Albumin (AA 34)
    • cell growth inhibiting protein 42
    • DKFZp779N1935
    • GIG20
    • GIG42
    • Growth inhibiting protein 20
    • growth-inhibiting protein 20
    • OTTHUMP00000220436
    • OTTHUMP00000220438
    • OTTHUMP00000220439
    • PRO0883
    • PRO0903
    • PRO1341
    • PRO1708
    • PRO2044
    • PRO2619
    • PRO2675
    • Serum albumin
    • UNQ696/PRO1341
    see all
  • Database links

    • Entrez Gene: 213 Human
    • Omim: 103600 Human
    • SwissProt: P02768 Human
    • Unigene: 418167 Human
    • Unigene: 592379 Human

    Associated products

    • SimpleStep ELISA kits

      • Human Albumin ELISA Kit (ab179887)

    Images

    • ab108788 Albumin Human ELISA kit
      ab108788 Albumin Human ELISA kit

      Concentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).

    • ab108788 Albumin Human ELISA kit
      ab108788 Albumin Human ELISA kit

      Standard curve with background signal subtracted (duplicates; +/- SD).

    • Typical Standard Curve for ab108788 Human Albumin ELISA kit
      Typical Standard Curve for ab108788 Human Albumin ELISA kit

      Representative Standard Curve using ab108788

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (36)

    Publishing research using ab108788? Please let us know so that we can cite the reference in this datasheet.

    ab108788 has been referenced in 36 publications.

    • Vasunilashorn SM  et al. Plasma and cerebrospinal fluid inflammation and the blood-brain barrier in older surgical patients: the Role of Inflammation after Surgery for Elders (RISE) study. J Neuroinflammation 18:103 (2021). PubMed: 33931093
    • Birukov A  et al. Normal-range urinary albumin excretion associates with blood pressure and renal electrolyte handling in pregnancy. Am J Physiol Renal Physiol 319:F1-F7 (2020). PubMed: 32463729
    • Miralda I  et al. Human Neutrophil Granule Exocytosis in Response to Mycobacterium smegmatis. Pathogens 9:N/A (2020). PubMed: 32075233
    • Subramani PA  et al. Plasmodium vivax liver stage assay platforms using Indian clinical isolates. Malar J 19:214 (2020). PubMed: 32571333
    • Pulsifer AR  et al. Redundant and Cooperative Roles for Yersinia pestis Yop Effectors in the Inhibition of Human Neutrophil Exocytic Responses Revealed by Gain-of-Function Approach. Infect Immun 88:N/A (2020). PubMed: 31871100
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-8 of 8 Abreviews or Q&A

    Abreview for Albumin Human ELISA kit

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    Abreviews
    abreview image

    Sample: Human Cell (HepG2 cells)
    Specification: HepG2 cells
    Amount of Sample: 0.2ug albumin standard µg
    Type of Kit Used: ELISA
    Positive control: Provided Albumin Standard
    Did you use the Abcam Kit protocol?: Yes

    Additional data
    Additional Notes: Recognized human albumin. Did not recognize bovine albumin in FBS.

    Abcam user community

    Verified customer

    Submitted Jul 03 2012

    Question

    We are looking to run a total protein assay on milk samples that have been diluted to be ran on this kit as well. Can you tell me if the Diluent N has any components that would interfere with a standard protein assay?

    Read More

    Abcam community

    Verified customer

    Asked on May 20 2015

    Answer

    Thank you for your inquiry. Ab108788 is validated for use with milk samples. The Diluent N does not contain BSA or casein but a BSA like protein. It does not interfere with human albumin measurement or cross react.

    Read More

    Abcam Scientific Support

    Answered on May 20 2015

    Question

    I would like to know if this kit is also good for plasma.

    Read More

    Abcam community

    Verified customer

    Asked on Apr 15 2015

    Answer

    The albumin ELISA ab108788 is designed to be able to detect the relatively low amounts (compared to serum and plasma) of albumin in the sample types listed on the datasheet: urine, saliva, milk, cerebrospinal fluid and cell culture supernatants. It will detect it in plasma and serum too but the samples would need to be diluted a great deal, as much as 1:250,000 or more, for the absorbance values to be within the range of the standards. A better choice is the Serum Albumin Human SimpleStep ELISA ab179887.

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Apr 15 2015

    Question

    Does the "10X Diluent Concentrate N" contain BSA or casein?

    Read More

    Abcam community

    Verified customer

    Asked on Dec 16 2014

    Answer

    The laboratory has confirmed that the "10X Diluent Concentrate N" does not contain BSA or casein. It contains other proteins that have no cross-reactivity with human albumin.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Dec 16 2014

    Question

    Is it possible to purchase Nx1 thinner for use with ab10878?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 02 2014

    Answer

    Yes, we added this diluent to our catalog: Diluent Concentrate (10x) ab172376.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Jun 02 2014

    Question

    Although this kit has not been tested in cell or tissue lysates I want to test it with cells removed from the matrix. Are there any lysis buffers that I should avoid that may interfere with the kit?

    Read More

    Abcam community

    Verified customer

    Asked on Sep 25 2013

    Answer

    While this kit as not been tested with tissue or cell lysates, in general, a lysis buffer containg a low concentration of triton-X-100 or SP-40 (less than 1 %) will not affect the assay.

    Read More

    Kevin Hanson

    Abcam Scientific Support

    Answered on Sep 25 2013

    Question

    Does ab108788 cross-react with horse serum albumin or BSA?

    Read More

    Abcam community

    Verified customer

    Asked on Jun 06 2013

    Answer


    The source of the kit ab108788 informed me that they have never tested this kit with horse serum. They have tested it with BSA and they did not see any significant cross-reactivity.

    Read More

    Abcam Scientific Support

    Answered on Jun 06 2013

    Question

    I run an assay yesterday using the Albumin Human ELISA kit (ab108788).

    Unfortunately, it was saturated. Is it possible to order the antibodies separately, that way I can re-run the experiment?

    Read More

    Abcam community

    Verified customer

    Asked on May 17 2013

    Answer

    The antibodies used in this kit are optimized for use in kit only. These are not available separately. We have other antibodies available in our catalogue that can be used to develop the assay e.g. ab10241/ ab2406, ab117455

    Read More

    Abcam Scientific Support

    Answered on May 17 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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