Overview

  • Product name

    Human Albumin ELISA Kit
    See all Albumin kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.7%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Cerebral Spinal Fluid
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 1.9 ng/ml
  • Range

    3.125 ng/ml - 200 ng/ml
  • Recovery

    97 %

  • Assay time

    3h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Human Albumin ELISA kit is designed for the quantitative measurement of albumin levels in urine, saliva, milk, cerebrospinal fluids and cell culture samples.


    An albumin specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an albumin specific biotinylated detection antibody is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of albumin captured in plate.


    Get results in 90 minutes with Human Albumin ELISA Kit (ab179887) from our SimpleStep ELISA® range.


    Please note: For both the 10X Diluent N Concentrate and 20X Wash Buffer Concentrate, please ensure to mix well by inversion before use to avoid precipitation.


     


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab108788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Concentration of albumin from differently diluted (in parenthesis) samples, demonstrating a linearity of 84-90% in CSF and 88-111% in milk (duplicates; +/- SD).

  • Albumin in HepG2 (diluted 1:50-200) and HeLa (undiluted) supernatants, heparin-treated plasma and serum (5e5-5e6), urine (100-500), milk (5000-30000), saliva (200-2000) was measured in duplicates (+/- SD).

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • Human albumin measured in various samples showing mass (microgram) per mL sample tested

  • Representative Standard Curve using ab108788

Protocols

References

This product has been referenced in:

  • Li L  et al. Establishment of gene-edited pigs expressing human blood-coagulation factor VII and albumin for bioartificial liver use. J Gastroenterol Hepatol N/A:N/A (2019). Read more (PubMed: 30884543) »
  • Saleh AF  et al. Extracellular vesicles induce minimal hepatotoxicity and immunogenicity. Nanoscale 11:6990-7001 (2019). Read more (PubMed: 30916672) »
See all 20 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Answer

Thank you for your inquiry. Ab108788 is validated for use with milk samples. The Diluent N does not contain BSA or casein but a BSA like protein. It does not interfere with human albumin measurement or cross react.

Read More

Question
Answer

The albumin ELISA ab108788 is designed to be able to detect the relatively low amounts (compared to serum and plasma) of albumin in the sample types listed on the datasheet: urine, saliva, milk, cerebrospinal fluid and cell culture supernatants. It will detect it in plasma and serum too but the samples would need to be diluted a great deal, as much as 1:250,000 or more, for the absorbance values to be within the range of the standards. A better choice is the Serum Albumin Human SimpleStep ELISA ab179887.

Read More

Question
Answer

The laboratory has confirmed that the "10X Diluent Concentrate N" does not contain BSA or casein. It contains other proteins that have no cross-reactivity with human albumin.

Read More

Question
Answer

Yes, we added this diluent to our catalog: Diluent Concentrate (10x) ab172376.

Read More

Answer

While this kit as not been tested with tissue or cell lysates, in general, a lysis buffer containg a low concentration of triton-X-100 or SP-40 (less than 1 %) will not affect the assay.

Read More

Question
Answer


The source of the kit ab108788 informed me that they have never tested this kit with horse serum. They have tested it with BSA and they did not see any significant cross-reactivity.

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Answer

The antibodies used in this kit are optimized for use in kit only. These are not available separately. We have other antibodies available in our catalogue that can be used to develop the assay e.g. ab10241/ ab2406, ab117455

Read More

Abreview for Albumin Human ELISA kit

Excellent Excellent 5/5 (Ease of Use)
Abreviews

Sample: Human Cell (HepG2 cells)
Specification: HepG2 cells
Amount of Sample: 0.2ug albumin standard µg
Type of Kit Used: ELISA
Positive control: Provided Albumin Standard
Did you use the Abcam Kit protocol?: Yes

Additional data
Additional Notes: Recognized human albumin. Did not recognize bovine albumin in FBS.

Abcam user community

Verified customer

Submitted Jul 03 2012

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