Overview

  • Product name
    Human Albumin ELISA Kit
    See all Albumin kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 8 2.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 8.8%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    210 pg/ml
  • Range
    0.31 ng/ml - 20 ng/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture extracts 81 81% - 82%
    Cell culture media 93 93% - 94%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human, Rhesus monkey
    Does not react with: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig
  • Product overview

    Albumin (ALB) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Albumin protein in human serum and plasma.


    The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.

  • Notes

    Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab179887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background subtracted data from triplicate measurements are plotted.

  • Observed ALB concentration in pooled donor normal Human serum (n=10). Mean Human serum values fall within expected normal reference ranges (ARUP/WHO).

  • Mean Human ALB values fall within expected normal reference ranges (WHO).

  • Animal serum from a variety of species were serially diluted and tested for cross reactivity using the Human Serum Albumin SimpleStep ELISA kit (ab179887). Data shows that ab179887 is >4 logs more sensitive and specific to Human serum albumin compared to other animal species.

Protocols

References

This product has been referenced in:
  • Smith JW  et al. Aflatoxin and viral hepatitis exposures in Guatemala: Molecular biomarkers reveal a unique profile of risk factors in a region of high liver cancer incidence. PLoS One 12:e0189255 (2017). Read more (PubMed: 29236788) »
  • Dickie AP  et al. The pharmacokinetics and metabolism of lumiracoxib in chimeric humanized and murinized FRG mice. Biochem Pharmacol 135:139-150 (2017). ELISA ; Human . Read more (PubMed: 28351678) »
See all 3 Publications for this product

Customer reviews and Q&As

Review the use of kit for CSF sample

Average Good 4/5 (Ease of Use)
Abreviews
The kit is easy to use. But I found that the variation between the duplicate is big for both standards and samples. I diluted the CSF samples at 3 different concentrations. It showed that 1:12000 dilution worked the best.

Abcam user community

Verified customer

Submitted Dec 20 2018

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