Human Albumin ELISA Kit (ab179887)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameHuman Albumin ELISA Kit
See all Albumin kits -
Detection methodColorimetric
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Precision
Intra-assay Sample n Mean SD CV% Overall 8 2.3% Inter-assay Sample n Mean SD CV% Overall 3 8.8% -
Sample typeCell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
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Assay typeSandwich (quantitative)
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Sensitivity210 pg/ml
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Range0.31 ng/ml - 20 ng/ml
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Recovery
Sample specific recovery Sample type Average % Range Cell culture extracts 81 81% - 82% Cell culture media 93 93% - 94% -
Assay time1h 30m
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Assay durationOne step assay
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Species reactivityReacts with: Human, Rhesus monkey
Does not react with: Mouse, Rat, Rabbit, Goat, Guinea pig, Hamster, Cow, Dog, Pig -
Product overview
Albumin (ALB) in vitro SimpleStep ELISA® (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Albumin protein in human serum and plasma.
The SimpleStep ELISA® employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. TMB substrate is added and during incubation is catalyzed by HRP, generating blue coloration. This reaction is then stopped by addition of Stop Solution completing any color change from blue to yellow. Signal is generated proportionally to the amount of bound analyte and the intensity is measured at 450 nm. Optionally, instead of the endpoint reading, development of TMB can be recorded kinetically at 600 nm.
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Notes
Albumin (ALB), the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. It also serves as a major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
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Tested applicationsSuitable for: Sandwich ELISAmore details
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PlatformMicroplate
Properties
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Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 10X Human Albumin Capture Antibody 1 x 600µl 10X Human Albumin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent CP 1 x 6ml Human Albumin Lyophilized Purified Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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FunctionSerum albumin, the main protein of plasma, has a good binding capacity for water, Ca(2+), Na(+), K(+), fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Major zinc transporter in plasma, typically binds about 80% of all plasma zinc.
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Tissue specificityPlasma.
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Involvement in diseaseDefects in ALB are a cause of familial dysalbuminemic hyperthyroxinemia (FDH) [MIM:103600]. FDH is a form of euthyroid hyperthyroxinemia that is due to increased affinity of ALB for T(4). It is the most common cause of inherited euthyroid hyperthyroxinemia in Caucasian population.
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Sequence similaritiesBelongs to the ALB/AFP/VDB family.
Contains 3 albumin domains. -
Post-translational
modificationsKenitra variant is partially O-glycosylated at Thr-620. It has two new disulfide bonds Cys-600 to Cys-602 and Cys-601 to Cys-606.
Glycated in diabetic patients.
Phosphorylation sites are present in the extracelllular medium.
Acetylated on Lys-223 by acetylsalicylic acid. -
Cellular localizationSecreted.
- Information by UniProt
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Alternative names
- alb
- ALBU_HUMAN
- Albumin (32 AA)
see all -
Database links
- Entrez Gene: 213 Human
- Omim: 103600 Human
- SwissProt: P02768 Human
- Unigene: 418167 Human
- Unigene: 592379 Human
Associated products
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SimpleStep ELISA kits
Applications
Our Abpromise guarantee covers the use of ab179887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Example of Albumin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Titration of NHS (pooled donor) within the working range of the assay.Background subtracted data from triplicate measurements are plotted.
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Observed Albumin concentration in pooled donor normal Human serumObserved ALB concentration in pooled donor normal Human serum (n=10). Mean Human serum values fall within expected normal reference ranges (ARUP/WHO).
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Observed Albumin levels in pooled donor normal Human plasmaMean Human ALB values fall within expected normal reference ranges (WHO).
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Cross Reactivity in Different Animal SerumsAnimal serum from a variety of species were serially diluted and tested for cross reactivity using the Human Serum Albumin SimpleStep ELISA kit (ab179887). Data shows that ab179887 is >4 logs more sensitive and specific to Human serum albumin compared to other animal species.
Protocols
References
This product has been referenced in:
- Smith JW et al. Aflatoxin and viral hepatitis exposures in Guatemala: Molecular biomarkers reveal a unique profile of risk factors in a region of high liver cancer incidence. PLoS One 12:e0189255 (2017). Read more (PubMed: 29236788) »
